摘要
为探讨外源抗坏血酸(AsA)对铝胁迫下水稻抗氧化损伤的效应,以滇优35号(杂交稻,粳稻)为试验材料,采用溶液培养法研究外源AsA对不同铝浓度(0、50、100、200、400μmol·L^-1)胁迫下水稻根尖H2O2含量及抗氧化酶活性等生理生化指标的影响。结果表明,水稻根尖氧化损伤程度随铝浓度的递增而加剧,与对照相比,400μmol·L-1铝胁迫可导致水稻根尖H2O2和MDA含量增加1.53和3.16倍,脯氨酸含量、SOD、POD、APX和CAT活性分别增加1.73、1.39、1.42、1.76和1.56倍,内源AsA含量减少0.53倍;而施用外源AsA处理的根尖H2O2和MDA含量只增加1.04和2.69倍,内源AsA含量减少0.46倍,脯氨酸含量增加1.96倍,SOD、POD、APX和CAT活性分别增加1.48、1.63、1.90和1.89倍。综上所述,外源AsA可通过增强渗透调节物质含量来维护质膜结构的完整性,增强抗氧化酶活性和内源AsA含量,降低根尖H2O2的积累和质膜过氧化程度,促进水稻在铝胁迫条件下的生长。本研究结果为进一步探索外源AsA缓解水稻铝毒的机理提供了一定的理论依据。
In order to investigate effect of exogenous ascorbic acid(AsA) on the antioxidant damage of rice under aluminum stress, the Dian Superior 35(Hybrid, Japonica) was used as the hydroponic test material to study the influence of exogenous AsA on the physiological and biochemical indicators such as the content of H2O2 and the activity of the antioxidant enzyme, etc. in the rice root tips under aluminum stress 0, 50, 100, 200, and 400 μmol·L-1, respectively. The results showed that the oxidative damage of rice roots was aggravated with the increase of aluminum concentration, meanwhile the content of H2O2 and MDA in the root tips increased by 1.53 and 3.16 folds, as well as the content of free proline, the activities of SOD, POD, CAT and APX increased by 1.73, 1.39, 1.42, 1.76 and 1.56 under 400 μmol·L^-1 aluminum stress, respectively. The content of endogenous AsA reduced by 53%. Compared to the control, the exogenous AsA treatment might result in the content of H2O2 and MDA in root tips only increased by1.04 and 2.69 folds, and the content of endogenous AsA reduced by 46%, while the content of free proline increased by 1.96 folds. The activities of SOD, POD, CAT and APX increased by 1.48, 1.63, 1.90 and 1.89 folds, respectively. The above results indicated that exogenous AsA could maintain the integrity of plasma membrane structure by enhancing the contents of osmoregulation substances, reduce the accumulation of H2O2 and the oxidation degree of plasma membrane by increasing the activity of antioxidant enzymes and content of endogenous AsA in roots, which could promote the growth of rice under aluminum stress. This result may provide a theoretical basis for further exploring the mechanism of exogenous AsA to alleviate aluminum toxicity in rice.
作者
周小华
周泽仪
李昆志
ZHOU Xiaohua;ZHOU Zeyi;LI Kunzhi(Kunming Cooperative Research Center for Pollution Prevention of Dianchi(Lake),Kunming University,Kunming,Yunnan 650214;College of Natural Resources and Environment,South China Agricultural University,Guangzhou,Guangdong 510000;Faculty of Life Science and Biotechnology,Kunming University of Science and Technology,Kunming,Yunnan 650500)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第10期2368-2375,共8页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金项目(31560351)
云南省高校联合基金项目(2018FH001-027)
昆明学院引进人才科研项目(YJL18009)。
关键词
水稻
铝胁迫
抗坏血酸
抗氧化酶
渗透调节物质
rice
aluminum stress
ascorbic acid
antioxidant enzyme
osmoregulation substances
作者简介
同第一作者\通讯作者:周小华,男,副教授,主要从事环境植物营养与保护研究。E-mail:zxh200008@126.com。
