摘要
目的:研究替莫唑胺联合姜黄素用药对C6胶质瘤细胞的抑制作用和诱导凋亡作用。方法:SRB法考察替莫唑胺联合姜黄素对C6细胞的抑制作用;流式细胞法检测联合用药对C6细胞的凋亡作用;激光共聚焦扫描显微镜观察联合用药对细胞的诱导作用及在细胞中的定位作用。结果:SRB法结果显示替莫唑胺联合姜黄素在48 h时对C6细胞的抑制率为(91.22±0.51)%,显著高于24 h的抑制率(83.66±0.18)%(P<0.05);流式细胞仪检测结果表明,替莫唑胺(10μmol/L)联合姜黄素(5μmol/L)用药时C6细胞的早期凋亡率为(33.15±0.79)%;通过激光共聚焦扫描显微镜观察,姜黄素联合替莫唑胺组诱导C6细胞的凋亡细胞数量多于游离药物组。结论:替莫唑胺联合姜黄素用药能够抑制C6细胞的生长,同时可诱导C6细胞的凋亡。
Objective To study the temozolomide combined with curcumin on the inhibitory effect and apoptosis of the C6 glioma cells. Methods The C6 glioma cells were treated with temozomide in combination with curcumin. The anti proliferation effect of liposomes on the C6 glioma cells was investigated by using the method of sulforhodamine B（SRB）. Flow cytometry was used to detect apoptosis of the C6 glioma cells. Confocal laser scanning microscope was used to observe apoptosis and location in the C6 glioma cells. Results The results of SRB assay showed that temozolomide in combination with curcumin inhibition rate were（91.22 ± 0.51）% in 48 h of the C6 glioma cells; Flow cytometry showed that the apoptosis rate were（33.15 ± 0.79）% with temozolomide（5 μmol / L）in combination with curcumin（10 μmol / L）. Laser scanning confocal scanning microscope indicated that the apoptosis of in the C6 glioma cells treated with temozolomide in combination with curcumin was more than that of free drug. Conclusion The temozolomide in combination of curcumin can inhibit the growth and induce apoptosis of the C6 glioma cells.
出处
《实用医学杂志》
CAS
北大核心
2016年第10期1564-1567,共4页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:81160396
81460540)
石河子大学杰出青年科技人才培育计划项目(编号:2012ZRKXJQ07)
新疆生产建设兵团社会发展科技攻关与成果转化计划项目(编号:2015AD007)
作者简介
通信作者：应雪E—mail：yingxue2011@qq．com
