摘要
目的:探讨TA2小鼠三阴性乳腺癌中乙醛脱氢酶1^+(ALDH1^+)和CD133^+表型的乳腺癌干样细胞在上皮间充质转化(EMT)发生中的作用,及对其生物学行为的影响。方法:使用流式细胞术分析TA2小鼠三阴性乳腺癌组织中ALDH1及CD133的表达量并分选出具有ALDH1^+、ALDH1-、CD133^+、CD133-表型的乳腺癌细胞,将其分别接种于TA2小鼠,根据细胞表型不同设置为ALDH1^+、ALDH1-,CD133^+、CD133-组,观察肿瘤生长情况,并制成组织切片,行三阴性乳腺癌中乳腺癌干细胞表面标记物ALDH1、CD133与EMT相关蛋白Twist1、E-cadherin和VE-cadherin的免疫组织化学检测,分析其表达差异。结果:乳腺癌干细胞标记物ALDH1及CD133在TA2小鼠三阴性乳腺癌中表达率分别为31.2%和6.5%。ALDH1^+、CD133^+组肿瘤生成能力明显强于ALDH1-、CD133-组。免疫组织化学结果显示ALDH1、Twist1、VE-cadherin在ALDH1^+组的表达明显高于ALDH1-组(均P<0.05),E-cadherin在ALDH1^+组的表达低于ALDH1-组(P<0.05)。CD133、Twist1、VE-cadherin在CD133^+组的表达明显高于CD133-组(均P<0.05),Ecadherin在CD133^+组的表达低于CD133-组(P<0.05)。结论:TA2小鼠三阴性乳腺癌中ALDH1^+和CD133^+表型的乳腺癌干样细胞可影响EMT相关蛋白的表达,并促进三阴性乳腺癌的形成。
Objective：To discuss the influence of ALDH1~＋and CD133~＋phenotypic breast cancer stem-like cells in TA2 triple negative breast cancer on promoting epithelial-mesenchymal transition（EMT） occurrence in TA2 mice with triple-negative breast cancer and on their biological behavior.Methods：Flow cytometry was performed to analyze the markers ALDH1 and CD133 in TA2 mice triple negative breast cancer and breast cancer stem-like cells with ALDH1~＋,ALDH1-,CD133~＋,and CD133-phenotypes,which were sorted out.Then,the TA2 mice were inoculated with sorted tumor cells according to cell type.The mice were divided into ALDH1~＋,ALDH1-,CD133~＋,and CD133-groups.The tumor-growing conditions were observed.A tumor tissue was sliced for the immunohistochemical testing of ALDH1-,CD133-,and EMT-related Twist1,E-cadherin,and VE-cadherin proteins.The expression difference of breast cancer stem cell surface markers ALDH1 and CD133 in triple-negative breast cancer and EMT-related proteins Twist1,E-cadherin,and VE-cadherin was analyzed.Results：The expression rates of breast cancer stem cell markers ALDH1 and CD133 in TA2 mice triple negative breast cancer were 31.2% and 6.5%,respectively.The tumor growth ability of TA2 mice from ALDH1~＋group was obviously stronger than that from ALDH1-group.The CD133~＋group was evidently stronger than CD133-group.The immunohistochemical results showed that ALDH1,Twist1,and VE-cadherin expression levels in the ALDH1~＋group were evidently higher than that in the ALDH1-group（all P〈0.05）.E-cadherin expression decreased（P〈0.05）.CD133-,Twist1,and VE-cadherin expression levels in CD133~＋group were higher than that in CD133-group（all P〈0.05）.Conclusion：In TA2 mice triple negative breast cancer,ALDH1~＋and CD133~＋phenotypic breast cancer stem-like cells may influence the expression of EMT-related proteins,and promote the formation of triple-negative breast cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2016年第8期324-328,共5页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金项目(编号:81230050)
天津医科大学科学基金(编号:2011ky34)资助
关键词
三阴性乳腺癌
上皮间充质转化
肿瘤干样细胞
ALDH1
CD133
流式细胞术
triple-negative breast cancer
epithelial-mesenchymal transition
cancer stem-like cells
aldehyde dehydrogenase 1
CD133
flow cytometry
作者简介
刘加蒙专业方向为肿瘤病理学。E-mail：paladinbbc@163．com
通信作者：孙保存sunbaocun@aliyun．com
