摘要
采用高效液相色谱法同时快速测定壮阳类保健品中的18种非法添加物。样品经乙腈(1+1)溶液超声提取后,提取液经以亚3微米填料的Agilent Poroshell 120 EC-C18色谱柱(50mm×4.6mm,2.7μm)分离,以(磷酸-三乙胺缓冲溶液)(pH 5.7)-甲醇-乙腈(70+15+15)混合液为流动相A、甲醇-乙腈(1+1)混合液为流动相B进行梯度洗脱,采用二极管阵列检测器,检测波长为230nm。18种添加物的检出限(3S/N)均为1.0 mg·kg^(-1),测定下限(10S/N)均为3.0mg·kg^(-1)。8种代表性添加物的线性范围为5.0~500 mg·L^(-1),加标回收率在88.2%~103%之间,测定值的相对标准偏差(n=6)在0.4%~1.6%之间。
HPLC was applied to simultaneous rapid determination of 18 additives in impotence healthy products.The 18 additives in samples were extracted ultrasonically with acetonitrile（1＋1）solution.The extract solution were separated on an Agilent Poroshell 120EC-C18column（50mm×4.6mm,2.7μm）filled with particles of sub-3μm,with a mixture of phosphoric acid-triethylamine（pH 5.7）buffer solution,methanol and acetonitrile as mobile phase A,and a mixture of methanol and acetonitrile as mobile phase B for gradient elution.The analysts were detected with DAD detector at the wavelength of 230 nm.The detection limits and the lower limits of determination of the 18 additives were 1.0 mg·kg^（-1） and 3.0 mg·kg^（-1） respectively.The linearity ranges of 8respective additives were 5.0-500 mg·L^（-1）.Recovery rates obtained by standard addition method were in the range of 88.2%-103%,and RSDs（n=6）were in the range of 0.4%-1.6%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2016年第3期249-254,共6页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
广东省省级科技计划项目(2013B090200059)
广东省中医药局课题(20132110)
广州市健康产品非法添加化学成分快速检测技术重点实验室项目(2013年)
作者简介
雷毅（1977-），男，云南昆明人，主任药师，主要从事食品药品检测工作。E-mail：Leiy04@qq．com.
