摘要
建立了高效液相色谱快速测定草本植物饮料中28种外源性药物和内源性成分的分析方法。样品经甲醇-水(70∶30,v/v)超声提取后,使用Thermo Accucore C18色谱柱(100 mm×4.6 mm,2.6μm),以甲醇、乙腈和20m m o l/L乙酸铵溶液(p H 4.2)为流动相进行梯度洗脱,流速为1.2 m L/m in,柱温为35℃,采用二极管阵列检测器,检测波长为254和220 nm,外标法定量。结果表明,28种成分在1~100 mg/L范围内线性关系良好,相关系数(r)均不小于0.999。液体样品和固体样品的检出限(LODs)分别为1~10和20~200 mg/kg。28种成分在液体和固体样品中的加标回收率分别为88.8%~118.6%和92.7%~112.3%,相对标准偏差(RSDs)分别为0.1%~6.7%和0.1%~6.4%。按上述方法检测草本植物饮料456批,检出阳性样品55批次,检出率为12.1%。该法简便快速、准确可靠,适用于草本植物饮料中28种成分的测定。
A high performance liquid chromatographic method for the determination of 28 exogenous medicines and endogenous components in the herbal drink was developed.The samples were extracted ultrasonically with methanol-water(70∶30,v/v),and the extracts were separated in a Thermo Accucore C18 column(100 mm×4.6 mm,2.6μm)with methanol-acetonitrile-20 mmol/L ammonium acetate solution(pH 4.2)as the mobile phases by gradient elution.The flow rate was 1.2 mL/min and the column temperature was 35℃.The detection wavelengths were 254 nm and 220 nm.Quantification analysis was performed by the external standard method.The result showed the compounds had a good linear relationship in the range of 1-100 mg/L,and the correlation coefficients(r)were not less than 0.999.The limits of detection(LODs)of the 28 compounds were 1-10 mg/kg in the liquid sample and 20-200 mg/kg in the solid sample.The average recoveries of the 28 compounds in the liquid and solid samples were in the ranges of 88.8%-118.6%and 92.7%-112.3%with the relative standard deviations(RSDs)of 0.1%-6.7%and 0.1%-6.4%,respectively.The method was applied to analyze 456 herbal drink samples,and 55 positive samples were found.The positive rate was 12.1%.The developed method was simple and reliable,and it was suitable for the determination of 28 components in the herbal drink.
作者
何嘉雯
温家欣
赖宇红
曹雅静
HE Jiawen;WEN Jiaxin;LAI Yuhong;CAO Yajing(Guangdong Institute for Drug Control,Guangzhou 510180,China)
出处
《色谱》
CAS
CSCD
北大核心
2018年第8期758-765,共8页
Chinese Journal of Chromatography
基金
广东省省级科技计划项目(2015A030401062)~~
关键词
高效液相色谱法
核壳色谱柱
非法添加药物
草本植物饮料
high performance liquid chromatography(HPLC)
fused core particle column
illegally added medicine
herbal drink
作者简介
通讯联系人.何嘉雯,E-mail:hjw7hjw@163.com.
