摘要
目的研究半边莲煎剂对肝癌H22荷瘤小鼠的抗肿瘤作用。方法建立肝癌H22荷瘤小鼠实体瘤模型,将24只小鼠分为3组,生理盐水组、半边莲煎剂组和5-氟脲嘧啶组,每组各8只。通过测定荷瘤小鼠的瘤重、抑瘤率观察半边莲煎剂对肿瘤生长的抑制作用,并采用免疫组化技术检测肿瘤组织中P27和BCL-2的表达情况。结果半边莲煎剂组瘤重与生理盐水组相比明显减小,差异有显著性意义(P<0.05)。半边莲有明显的抑瘤作用,其抑瘤率为39.48%。P27和BCL-2在3组中均呈阳性表达,半边莲煎剂组的P27表达增强,BCL-2表达减弱,与生理盐水组比较,差异均有显著性意义(P<0.05);而半边莲煎剂组与5-氟脲嘧啶组比较,差异无显著性意义(P>0.05)。结论半边莲煎剂具有明显的抑瘤作用,其作用机制可能与上调P27和下调BCL-2表达有关。
Objective To study the anti- tumor effects of Banbianlian on H22 hepatocelluar carcinoma- bear mice.Methods H22 hepatocelluar carcinoma- bear mice model was established; 24 mice were divided equally into three groups：normal saline treated group,Banbianlian treated group and 5- Fu treated group. The tumor was removed and measured.P27 and BCL- 2 expression in the tumor tissues were examined in immunohistochemistry. Results Banbianlian inhibited the tumor growth of hepatocelluar carcinoma- bear mice with the inhibitory rate 39. 48%. The expression of P27 and BCL- 2 in three groups were positive. P27 expression was stronger,while BCL- 2 expression was weaker in Banbianlian treated group than those in normal saline treated group（ P 〈0. 05）. Two protein expression were not significantly different between Banbianlian treated group and 5- Fu treated group（ P〉 0. 05）. Conclusion Banbianlian could inhibit tumor growth,which could be related to the abnormal expression of P27 and BCL- 2.
出处
《大连医科大学学报》
CAS
2016年第1期20-23,共4页
Journal of Dalian Medical University
作者简介
刘晓宇（1984-），女，辽宁大连人，主治医师。E—mail：liuxiaoyu528@163．com
通信作者：张红，教授。E—mail：zh19600901@163．com
