摘要
以番木瓜茎段为材料,建立无菌组培快繁体系,比较不同激素组合对腋芽初代培养的影响;以继代第2代的芽为试材,采用L9(34)正交试验设计,探讨6-BA、KT、NAA和GA对番木瓜增殖培养的影响,优化增殖培养基;进一步研究番木瓜的生根技术。结果表明:(1)番木瓜外植体经预处理后,用75%酒精表面灭菌20 s,再用0.1%Hg Cl2浸泡25 min的消毒效果较好,污染率为16.67%,萌芽率为77.94%,褐化率为13.33%。(2)MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+蔗糖30 g/L为腋芽初代诱导的最佳培养基,腋芽萌芽率达76.67%。(3)正交试验设计的9组培养基中,最利于增殖和株高的均为MS+6-BA 0.5 mg/L+KT 1.5 mg/L+NAA 0.5 mg/L+GA 0.6 mg/L,30 d的增殖系数为6.87、株高为3.31 cm;对番木瓜继代苗增殖系数和株高的影响大小均表现为6-BA>GA>KT>NAA。(4)增殖培养前期的最佳培养基为MS+6-BA 0.5 mg/L+KT 0.5 mg/L+NAA 0.5 mg/L+GA 0.6 mg/L,中后期的最佳培养基为MS+6-BA 0.5 mg/L+KT 0.5 mg/L+NAA 0.3 mg/L+GA 0.6 mg/L。(5)1/2MS+IBA 0.5 mg/L+NAA 0.1mg/L+蔗糖30 g/L+AC 0.1%的生根效果最好,生根率达65%,每株根数为4.68条,生根苗移栽成活率达60%以上。
Stem segments of papaya were used as explants to establish the aseptic system and compare the effects of different hormone combinations on the primary culture of axillary buds. The second generations of buds were used as test materials in the subculture, to examine the effects of 6-BA, KT, NAA and GA on the proliferation culture of papaya by using the orthogonal experiment design L9 （34） , and the multiplication medium was optimized. And the rooting techniques of papaya were studied too. The results showed that： （1）Rapidly immersed with 75% alcohol for 20 s plus 0.1% HgC12 for 25 min after the pretreatment was the better disinfection method with the contamination rate of 16.67%, the germination rate of 77.94%, and the browning rate of 13.33%. （2）The best medium for primary culture was MS ＋ 6- BA 0.5 mg/L ＋ NAA 0.1 mg/L ＋ sucrose 30 g/L with the induction rate of axillary buds up to 76.67%. （3） The most suitable medium for proliferation and plant height was MS ＋ 6-BA 0.5 mg/L ＋ KT 1.5 mg/L ＋ NAA 0.5 mg/L ＋ GA 0.6 mg/L, the multiplication coefficient was 6.87 and the plant height was 3.31 cm after 30 d, and the effects of different hormones on the multiplication coefficient and plant height of papaya were all ranked in the following order： 6-BA 〉 GA 〉 KT 〉 NAA. （4） The best medium for early proliferation culture was MS ＋ 6-BA 0.5 mg/L ＋ KT 0.5 mg/L ＋ NAA 0.5 mg/L ＋ GA 0.6 mg/L, and the best medium for middle and late proliferation culture was MS ＋ 6-BA 0.5 mg/L ＋ KT 0.5 mg/L ＋ NAA 0.3 mg/L＋GA 0.6 mg/L. （5）The best rooting medium was 1/2MS＋IBA 0.5 mg/L＋NAA 0.1 mg/L＋sucrose 30 g/L＋AC 0.1% with the rooting rate up to 65%, the root number per plant up to 4.68, the survival rate of rooting seedlings over 60%.
出处
《广东农业科学》
CAS
2015年第11期59-64,F0002,共7页
Guangdong Agricultural Sciences
基金
国家林业局推广项目(2012[065])
关键词
番木瓜
组织培养
快繁效率
影响因子
正交试验
papaya
tissue culture
rapid propagation efficiency
influence factor
orthogonal test
作者简介
叶维雁（1988-），男，在读硕士生，E—mail：flyingywy@163．com
通讯作者：王连春（1973-），男，博士，副教授，E—mail：Kaixianlc@126．com
