摘要
从海南岛感病的番木瓜(Carica papaya L.)叶片中分离和纯化番木瓜环斑病毒(PRV),以Olig(dT)(18 bp)为引子,合成 cDNA第一链,用PCR的方法扩增该病毒外壳蛋白基因并克隆在BluscriptKS载体上。经酶切图谱分析、DNA全序列测定证明该基因是完整的。将此序列与国内外发表的PRV外壳蛋白基因的序列比较,同源率根高,PRV外壳蛋白基因的N端是高突变区。
Papaya rinspot virus (PRV) was isolated and purified from infected papaya (Carica papaya L.)leaves in Hainan Island. The first strand of cDNA was synthesized with Olig(dT)as a primer. The coat protein gene of PRV was obtained by PCR techniques with primers synthesized accoding to the DNA sequence of PRV-P. Full length of cDNA clone encoding coat protein of PRV was se- quenced and analysed. The result shows that the strain of PRV we isolated contains 881 nu- cleotides with 287 amino acids. Sequences among several strains of PRV were compared and it in- dicates an over 90% homology in DNA sequence of PRV-G the strain we isolated with PRV-P and PRV-YS. Highly convered sequence was located in carboxyl end and interestingly highly variable region was in N-terminal.
关键词
番木瓜
环斑病毒
外壳蛋白基因
Papaya
Papaya rinspot virus
Coat protein gene sequence
