摘要
以宁夏常见水生植物香蒲(Typha angustifolia)为材料,进行香蒲组培快繁技术研究.结果表明:香蒲组织培养对激素种类及其质量浓度有较广泛的适应性,但在不同的培养基下,其快繁效率不同.最佳生根培养基为1/2 MS+NAA 0.5 mg/L+6-BA 0.04 mg/L和1/2 MS+IBA 0.1 mg/L,生根率达100%,生根4~7条.经过多次试验最终确定最佳外植体为茎尖,最佳培养基为1/2 MS基本培养基.
The rapid propagation technique of Typha angustifolia is developed through tissue culture. The results shows that tissue culture of Typha angustifolia is widly adapted to hormone types and concentration.The best explants for tissue culture is shoot tip, and an optimum medium for culturing the tips with 1/2 MS culture should include other chemicals, such as 6-BA,IBA and NAA. We conclude that Typha angustifolia adapts well to different hormones at various concentrations, including either6-BA(0.02~0.10 mg/L), NAA(0.3~0.7 mg/L) or IBA(1.0 mg/L). The types of the culture media determine the culture efficiency. It is concluded that the best media includes either 1/2 MS plus NAA(0.5 mg/L) and 6-BA(0.04 mg/L), or 1/2 MS plus IBA(0.1 mg/L). In which the rooting rate reaches 100%, that is, the roots for each tissue pieces are 4 to 7.
出处
《农业科学研究》
2014年第2期79-82,共4页
Journal of Agricultural Sciences
基金
国家级大学生创新实验项目(02zh07-01)
关键词
香蒲
组织培养
快繁技术
Typha angustifolia
tissue culture
rapid propagation
