摘要
建立同时测定马黛茶多酚中4种有效成分——新绿原酸、绿原酸、异绿原酸A、异绿原酸C的加压毛细管电色谱方法。以乙腈、甲醇和磷酸（p H 2.5,2 mmol/L）为流动相,流速0.07 m L/min,分离电压-10 k V,检测波长325 nm。测定和比较了3种马黛茶产品中4种成分的含量,4种成分分别在0.072~1.2、0.048~0.8、0.072~1.2、0.17~2.8 mg/m L内线性关系良好（r2〉0.999）,精密度分别为1.10%、2.12%、2.98%和2.32%,重复性RSD分别为1.10%、2.12%、2.98%、2.20%,平均回收率分别为98.86%、99.03%、99.48%和99.21%。该方法灵敏、准确,可以用于马黛茶中绿原酸类活性物质分析。与HPLC比较,实验中所用有机试剂、样品用量均远低于普通HPLC检测用量,对于马黛茶多酚的质量控制及其大批量检测具有较大的可行性。
To establish an analytical method of simultaneous determining of neochlorogenic acid, chlorogenicac- id,isochlorogenic acid A,isochlorogenic acid C-four bioactive compounds from Mate Tea （ Ilex paraguariensis） using- pCEC （pressurized capillary eleetrochromatogaphy）. The mobile phase was composed of methanol and acetonitrilecon- taining phosphoricacid（pH 2.5,2 mmol/L）with the flow rate of 0.07 mL/min. Chromatography was monitored at wavelength 325 nm and the separation voltage was - 10 kV. The contents of four phenolic components in 3 batches of samples collected from different typeswere determined and compared. The correlation coefficients between concentra- tions and chromatographic peak areas of neochlorogenic acid,chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C wereover 0. 999 and the linage ranges were 0. 072 - 1.2,0. 048 ~ 0.8,0. 072 - 1.2,0.17 ~ 2.8mg/mL, re- spectively. The precisionwas 1. 10% , 2. 12% , 2. 98% and 2. 32% , respectively. The repeatabilitywas 1. 10% , 2.12% ,2.98% , 2. 20% , respectively. The average recoveries of the four compounds were 98. 86% , 99.03% , 99.48% and 99.21% , respectively. Themethod is sensitive, accurate, and can be used for the analysis of phenolic compounds from Mate tea（Ilex paraguariensis）. Compared with HPLC, organic reagents used in the experiment, the dosage were much lower than normal dosage of HPLC detection. The method has great feasibility in the quality control of polyphenols and their mass composition detection.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第1期234-238,共5页
Food and Fermentation Industries
基金
国家重大科学仪器设备开发专项
(2011YQ15007208)
国家重大专项(2014zx09201001-012)
"十二五"大平台子课题(X-2-3)
上海市科技支撑项目(12431901102)
国家重大新药创制(2012ZX09301002-003)
关键词
马黛茶
多酚
加压毛细管电色谱法
定量分析
Mate tea（llex paraguarienses）
phenoliccompounds
pressurized capillary electrochromatogaphy
quanti-fication
作者简介
硕士研究生
（胡海峰研究员为通讯作者。E-mail：haifenghu88@163．com）。
