摘要
建立了高效液相色谱测定黑米酒中11种酚类化合物的方法.采用VenusilC18色谱柱（250mm×4.6mm,5μm）分离,流动相为50％乙腈和超纯水,分别加1％乙酸,采用梯度洗脱,流速为1.0mL/min,进样量20μL,柱温30℃,检测波长260hm、320hm.各组分的质量浓度与其峰面积具有良好的线性关系,相关系数均大于0.996,且11种酚类物质在45min内得到了较好分离.方法回收率为83.5％～105.1％,相对标准偏差为1.3％～3.3％.该方法可用于米酒中黄酮、酚酸类化合物的定量分析.
Simultaneous determination of 11 phenolic constituents in black rice wine was established by HPLC method.Venusil C18 （250mm×4.6mm,5μm） column was used to seperate the phenolic compounds.Gradient elution was employed with a moble phase consisting of 50％ acetonitrile and ultrapure water.1％ acetic acid was added at a solvent flow rate of 1.0 mL/min with injection volume of 20μL.The 11 flavonoids and phenolic acids were identified at 30℃,at 260nm and 320nm,respectively.The phenolic compounds were separated well in 45 min,and method showed good linear correlations between the concentration and peak area,and the correlation coefficient was greater than 0.996.The average recoveries were 83.5％～ 105.1％,with relative standard deviations 1.3％～3.3％.The method can be applied to the quantitative analysis of flavonoids and phenolic acids in rice wine.
出处
《中国酿造》
CAS
2014年第2期134-137,共4页
China Brewing
基金
西北农林科技大学大型仪器设备新功能开发项目(dysb130205)
关键词
高效液相色谱
黑米酒
黄酮
酚酸
米酒
检测
HPLC
black rice wine
flavonoids
phenolic acids
rice wine
determination
作者简介
姜莉（1968-）,女,高级实验师,硕士,主要从事食品化学与分析的教学及研究工作.
通讯作者：王玉堂（1981-）,男,讲师,博士,主要从事食品化学与分析的教学及研究工作.
