摘要
为了研究植物生长调节剂对橡胶树不定芽诱导的影响,笔者以橡胶树优良无性系‘云研73-477’花药体胚植株为外植体,采用正交试验法研究了6-BA、NAA、KT、GA3对橡胶树不定芽诱导的影响。结果表明,4种激素对茎尖不定芽诱导率的影响差异不显著,对繁殖系数的影响差异达显著水平,其主次顺序为:NAA>6-BA>KT>GA3,优组合为1.0 mg/L 6-BA+0.2 mg/L NAA+0.2 mg/L KT+0.4 mg/L GA3;6-BA、NAA、GA3对茎段芽繁殖系数的影响差异达显著水平,KT则不显著,NAA、GA3对死亡率的影响差异达极显著水平,其他2种激素则不显著。茎段不定芽诱导的优组合为1.5 mg/L6-BA+0.1 mg/L NAA+0.2 mg/L KT+0.2 mg/L GA3。
The paper aims to study the effects of plant growth regulators on induction of adventitious buds ofHevea brasiliensis Mull.Arg. Apical buds and stems of Hevea brasiliensis clone Yunyan73-477 were used asexplants, the effects of 6-BA, NAA, KT and GA3 on induction of adventitious bud were studied by orthogonalexperiment. The results showed that the effect of each factor on the coefficient of breeding of apical buds was NAA〉6-BA〉KT〉GA3, and 1.0 mg/L 6-BA+0.2 mg/L NAA+0.2 mg/L KT +0.4 mg/L GA3 was the optimalcombination; the effects of four factors except KT were significantly different at 0.05 level on the coefficient ofbreeding of stem. The effect of NAA and GA3 were significantly different at 0.01 level on the dead rate of stem,but 6-BA and KT were not. 1.5 mg/L 6-BA+0.1 mg/L NAA+0.2 mg/L KT+0.2 mg/L GA3 was the optimalcombination on induction of adventitious bud of stems.
出处
《中国农学通报》
2015年第12期224-229,共6页
Chinese Agricultural Science Bulletin
基金
云南省应用基础研究自筹经费项目"云南优良橡胶树品种幼态芽条繁殖技术的研究"(2013FZ169)
2014云南省热带作物科技创新体系建设资金项目"橡胶树育种材料及方法创新"(RF2014)
云南省科研院所技术开发研究专项资助项目(2013DC013)
关键词
橡胶树
植物生长调节剂
不定芽
诱导率
Hevea brasiliensis
plant growth regulators
adventitious bud
inductive rate
作者简介
桂明春,女,1985年出生,云南普洱人,研究实习员,硕士,研究方向:橡胶树组织培养和遗传育种。通信地址:666100云南省西双版纳州景洪市宣慰大道99号云南省热带作物科学研究所,Tel:0691-2120332,E-mail:564863423@qq.com。
通讯作者:梁国平,男,1965年出生,湖南醋陵人,高级农艺师,本科,研究方向:橡胶树组织培养与遗传育种。通信地址:666100云南省西双版纳州景洪市宣慰大道99号云南省热带作物科学研究所,Tel:0691-2120332,E-mail:gPliang213@126.com。