摘要
目的:探讨自噬相关蛋白Beclin1、LC3和P62在进展期胰腺癌组织中的表达及临床意义.方法:应用免疫组织化学法检测64例胰腺癌组织及相应癌旁正常组织中自噬相关蛋白Beclin1、LC3和P62的表达情况;分析自噬蛋白表达水平与胰腺癌临床病理特征的关系.结果:免疫组织化学法检测提示Beclin1蛋白在胰腺癌组织及癌旁正常组织中阳性表达率分别为28.1%(18/64)、90.6%(58/64),LC3蛋白阳性表达率分别为34.4%(22/64)、75%(48/64)P 6 2蛋白表达阳性率分别为7 5%(4 8/6 4)、4 6.9%(3 0/6 4),均P<0.0 5;胰腺癌组织中Beclin1与LC3的表达水平呈正相关(r=0.572P<0.05),Beclin1、LC3的表达与肿瘤分化、TNM分期相关(P<0.05),Beclin1、LC3和P62的表达均与性别、年龄、有无门脉浸润、淋巴结转移无明显相关(P>0.05).结论:自噬相关蛋白Beclin1、LC3和P62在胰腺癌组织中的异常表达及Beclin 1与LC3两者表达呈正相关提示自噬活动下降可能参与了进展期胰腺癌的发展.
AIM： To investigate the expression of Beclin1, LC3 and P62 in advanced pancreatic cancer and to analyze their correlation with clinical and pathological parameters of pancreatic cancer.METHODS： Immunohistochemistry was used to detect the expression of Beclin1, LC3 and P62 in 64 pancreatic cancer specimens and matched tumor-adjacent noncancerous tissues.RESULTS ： The positive rates of Beclin 1 [28.1%（18/64） vs 90.6%（58/64）, P〈 0.05] and LC3 [34.4%（22/64） vs 75%（48/64）, P 〈0.05] expression were significantly lower, and that of P62 expression [75%（48/64） vs 46.9%（30/64） P 〈0.05] was significantly higher in pancreatic cancer than in matched tumoradjacent noncancerous tissues. There was a significant correlation between Beclin1 and LC3 expression in pancreatic cancer（r = 0.572, P〈 0.05）. The expression of Beclin1 in pancreatic cancer was associated with tumor differentiation and stage（P 〈0.05）, while Beclin1, LC3 and P62 expression in pancreatic cancer had no significant correlation with gender, depth of tumor invasion, or lymph node metastasis（P 〉0.05）. CONCLUSION： The abnormal expression of Beclin1, LC3 and P62 may be related to the carcinogenesis of pancreatic cancer.
出处
《世界华人消化杂志》
CAS
2015年第2期318-323,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助资助项目
Nos.81001103
81472325
河南省卫生科技创新型人才工程基金资助项目
No.4107~~
作者简介
张创杰，在读硕士，主要从事消化系疾病的研究．
通讯作者：周琳．副教授，硕士生导师，450052，河南省郑州市二七区建设东路1号，郑州大学第一附属医院消化内科．zl372@126．com,电话：0371—66862062
