摘要
为了全面地了解DNA指纹在品种鉴定上的利用现状,更重要的是促进DNA指纹技术切实有效的应用于植物品种鉴定,本研究归纳了目前开发出的可用于植物品种鉴定的DNA指纹技术,指出已有的DNA指纹品种鉴定技术的优势与不足,总结了DNA指纹应用于品种鉴定的分析措施,并认为受限于缺乏可以将DNA指纹转化为植物鉴定信息的理想措施,所以DNA标记还未真正有效地应用于生产实践中,因此提出一种基于DNA指纹的人工绘制品种鉴定简图(MCID)法是很重要的,本研究也重点阐述了MCID法的实施过程。
In order to deeply understand the status of the usage of DNA fingerprinting in the plant cultivars identification, more importantly, to promote DNA fingerprinting techniques＇ application in plant cultivars identification, this paper summarized the DNA fingerprinting techniques developed currently that used to identify plant cultivars and pointed out the advantages and disadvantages of the existing DNA fingerprinting identification techniques. The analysis measures applying to the DNA fingerprinting in cuhivars identification were also reviewed. Because of the lack of the ideal measures, the techniques limited the transformation between DNA fingerprinting and plant identification information. Therefore, DNA markers have not really effectively applied in production practice. As a identification diagram （MCID） method based on implementation of MCID. result, it was very important to propose a manual cultivar the DNA fingerprinting. This article also focuses on the implementation of MCID.
出处
《中国农学通报》
CSCD
2014年第30期234-240,共7页
Chinese Agricultural Science Bulletin
基金
科技部科技基础专项项目"中国优势产区落叶果树农家品种资源调查与收集子项目"(2012FY110100-3)
关键词
品种鉴定
分子标记
人工绘制植物品种鉴别图
cuhivar identification
molecular markers
manual cultivar identification diagram
作者简介
朱旭东，男，1988年出生，湖北黄冈人，硕士研究生，主要从事果树基因组学和分子生物学研究。通信地址：210095江苏省南京市卫岗一号南京农业大学园艺学院，Tel：025-84399069，E-mail：2012104017@najau.edu．cn。
通讯作者：房经贵，男，1968年出生，山东莒县人，教授，博导，博士，研究方向：果树遗传育种与基因组学。通信地址：210095江苏省南京市卫岗一号南京农业大学园艺学院，Tel：025-84399069，E-mail：fanggg@njau．edu．cn。
