摘要
葡萄品种繁多,表型现象不明显,同名异物或同物异名现象时有发生,品种的精准鉴定异常重要。本文利用8对引物的SSR指纹图谱通过NTSYS-2.10e软件对来源于不同国家及地区的72个酿酒葡萄品种进行了聚类分析,所得的聚类树状图直观反映了各品种间的遗传关系。在遗传相似系数为0.52左右时,可以分为4个类群。同时也发现聚类结果无法显示特定的引物和多态性标记等可用于品种鉴定参考的信息,限制了分子标记在品种鉴定中的实际应用。本文同时利用基于SSR分子标记的人工绘制植物品种鉴别图法(MCID),通过筛选出的6对引物的多态性指纹图谱,绘制了这些品种的鉴定图(CID),成功将72个酿酒品种进行区分。从CID图上可以找到任意2个或多个品种间进行区分与鉴定的引物以及依据的多态性条带。这对酿酒葡萄品种鉴定、新品种保护及促进酿酒葡萄产业的持续发展具有重要作用。
There are many varieties of grapes with inapparent phenotype,and the homonymous phenomena occur from time to time,and the accurate identification of grape varieties is very important.72 wine grape cultivars from different countries and regions were clustered by NTSYS-2.10e using 8 pairs of primers SSR fingerprints in this paper.The clustering tree graph obtained directly reflected the genetic relationship among the cultivars.When the genetic similarity coefficient was about 0.52,they could be divided into four groups.At the same time,it was found that clustering results could not display specific primers and polymorphic markers,which could be used as reference information for cultivar identification,thus limiting the practical application of molecular markers in cultivar identification.Then the polymorphic fingerprints of six pairs of primers were screened out by the manual cultivar identification diagram (MCID) based on SSR molecular markers,and the cultivar identification diagram (CID) of these varieties was drawn which successfully distinguished 72 wine grape varieties.Primers and polymorphic bands for identification and differentiation between any two or more cultivars could be found on the CID map,which might be helpful in the identification of wine grape varieties,the protection of new varieties and the sustainable development of wine grape industry.
作者
傅伟红
魏新科
葛孟清
刘崇怀
房经贵
FU Weihong;WEI Xinke;GE Mengqing;LIU Chonghuai;FANG Jinggui(College of Horticulture,Nanjing Agricultural University/Fruit Crop Variety Improvement and Seedling Propagation Engineering Center of Jiangsu Province,Nanjing 21095,China;Zhengzhou Fruit Research Institute,CAAS,Zhengzhou 450009,China)
出处
《中外葡萄与葡萄酒》
2019年第5期1-7,共7页
Sino-Overseas Grapevine & Wine
基金
江苏省农业科技自主创新项目（CX（18）2008）
江苏现代农业（葡萄）产业技术体系JATS[2018]279
作者简介
傅伟红(1996-),女,浙江衢州人,硕士研究生,研究方向为果树分子生物学。E-mail:2018804144@njau.edu.cn;通信作者:刘崇怀,E-mail:liuch1965@126.com;通信作者:房经贵,E-mail:fanggg@njau.edu.cn.
