摘要
DNA指纹技术的发展大体分为三个阶段:第一代的分子标记是以Southern杂交为基础的RFLP,第二代分子标记是以PCR为基础的各种DNA指纹标记,第三代分子标记是以单核苷酸多态性为基础的SNP。本文概述了品种鉴定技术的研究与应用进展,重点介绍了用于棉花品种鉴定的四种主要的分子标记技术:RFLP、RAPD、AFLP和SSR,对每一种分子标记的技术原理、在棉花品种鉴定上的应用研究状况及存在的优缺点进行了具体阐述。通过比较分析,提出了SSR标记适用于棉花品种鉴定的独特优越性。并对基于SSR分子标记技术构建我国棉花品种DNA指纹库的重要意义与必要性进行了分析。
There are three phases of DNA fingerprint technology based on molecular marker：the first is RFLP based on Southern blot,the second are several markers based on PCR and the third is SNP（single nucleotide polymorphism）. Research and application of variety identification technology was discussed,four main molecular marker technologies applied in cotton variety identification were introduced in detail as follow ：RFLP, RAPD, AFLP and SSR. Technology principle, research and application status, advantage and disadvantage about each molecular marker applied in cotton variety identification were expatiated. As compared,the viewpoint was put forward：SSR marker was the most appropriate molecular marker in cotton variety identification, and the necessity of construction of Chinese cotton DNA fingerprint database based on SSR marker technology was analyzed.
出处
《棉花学报》
CSCD
北大核心
2009年第4期330-334,共5页
Cotton Science
基金
中央级公益性科研院所基本科研业务专项(SJB0805)
关键词
棉花
分子标记
品种鉴定
DNA指纹库
cotton
molecular marker
variety identification
DNA fingerprint database
作者简介
匡猛（1982-），男，硕士，研究实习员，kuangm@cricaas．com．cn
