摘要
利用独角莲水提物对酪氨酸酶抑制活性的测试,研究独角莲水提物的提取工艺.通过L9(34)正交试验法研究了料液质量比、提取时间、提取温度和提取次数对独角莲水提物抑制酪氨酸酶活性的影响.结果表明:独角莲水提物的最佳提取工艺为料液质量比1∶15,提取时间3 h,提取温度80℃,提取次数1次,在此条件下测定独角莲水提物对酪氨酸酶的抑制率,最高为46.93%.
The extraction technology of water extracts from Typhonium giganteum Engl. was studied and the effect on tyrosinase activity was investigated. The influence of material-liquid ratio, extraction time, extraction temperature and extraction times on inhibitory activity against tyrosinase was studied through L9 （ 34 ） orthogonal experiments. The effects on tyrosinase activity was dertermined by Folin-reagent method. The optimum extraction condition was material-liquid ratio 1：15 （weight ratio）, extraction time 3 h, extraction tempreture 80 ℃, and extraction for 1 time. At this condition, water extracts from Typhonium giganteum Engl. had the highest inhibitoty effects on tyrosinase, which was 46. 93 %.
出处
《沈阳化工大学学报》
CAS
2014年第3期231-233,共3页
Journal of Shenyang University of Chemical Technology
基金
辽宁省大学生科技创新项目(201210149004)
辽宁省教育厅项目(L2012146)
关键词
独角莲
水提物
酪氨酸酶
Typhonium giganteum Engl.
water extracts
tyrosinase
作者简介
宋艳玲（1977-）,女,吉林通化人,副教授,博士,主要从事天然产物药物化学方面的研究.
