摘要
为提高贯叶连翘不定根提取物抑制酪氨酸酶活性的效果,该试验对不定根提取时的提取溶剂、提取物浓度和提取时间、提取温度、提取次数及甲醇浓度等条件进行了优化,以探明对酪氨酸酶活性抑制的最优提取工艺。结果表明:不定根甲醇提取物对酪氨酸酶的抑制率高于乙醇和水提物,提取物浓度为800μg/mL时,抑制效果好于其它浓度。在提取时间1 h,提取温度70℃,提取次数4次和甲醇浓度为80%条件下提取的贯叶连翘不定根提取物对酪氨酸酶活性的抑制率达到最高,为90.2%。
To enhance the inhibitory effect of extracts from Hypericum perforatum adventitious roots on tyrosinase activity, the present study optimized extraction conditions including the extraction solvent, concentration, duration, temperature, times and methanol concentration,and verified the optimal extraction process on inhibition of tyrosinase activity. The results showed that the methanol was the better solvent for the extraction of adventitious roots compared with ethanol and water, and the extraction concentration of 800 μg/mL was more favorable. The optimal extraction conditions were: 80% methanol for 1 h at 70 ℃ for 4 times, the inhibitory rate of on tyrosinase activity was the highest and reached 90.2%.
作者
孙浩丁
郝悦君
安晓丽
廉美兰
朴炫春
SUN Haoding;HAO Yuejun;AN Xiaoli;LIAN Meilan;PIAO Xuanchun(Agricultural College of Yanbian University,Yanji Jilin 133002,China)
出处
《延边大学农学学报》
2019年第3期23-28,共6页
Agricultural Science Journal of Yanbian University
基金
国家自然科学基金(31660080)
关键词
贯叶连翘
不定根
提取条件
酪氨酸酶活性
Hypericum perforatum
adventitious roots
extraction process
tyrosinase activity
作者简介
孙浩丁(1995—),男,吉林长春人,在读硕士,研究方向为植物组织培养及生物反应器应用;通信作者:朴炫春,E-mail:nyypxc@ybu.edu.cn。
