摘要
目的探讨白蒺藜中酪氨酸酶抑制剂的微波提取工艺。方法以提取物的产率和对酪氨酸酶的相对抑制率为评价指标,采用正交试验和单因素试验,结果水为提取剂、水与白蒺藜的质量比10∶1、微波450 W提取(5 min×2)为最佳提取工艺。提取物(干基)产率为16.6%,对酪氨酸酶的相对抑制率为43.8%。结论与直接加热提取法比较,微波辅助提取能缩短提取时间,并能提高提取物产率和对酪氨酸酶的相对抑制率。
OBJECTIVE To establish the microwave- assisted extraction（MAE）technology of inhibitor against tyrosinase from Tribulus terrestris L.METHODS The single factor method and orthogonal method were used in the experiment.The yield and the relative inhibitory activity against tyrosinase of the extracts were used as evaluating indexes. RESULTS It was concluded that the optimal condition was twice microwave radiation （450 W） for 5 min with a mass ratio of water to material of 10：1 .The dry extract of Tribulus terrestris L. with 16.6% yield and 43.6% relative inhibitory activity against tyrosinase was obtained. CONCLUSION Compared with direct-heating extraction, MAE can shorten extracting time, and improve the yield and the relative inhibitory activity against tyrosinase of the extracts.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2006年第1期50-52,共3页
West China Journal of Pharmaceutical Sciences
基金
广东佛山市产学研专项资金项目(2003A36)
广东轻工职业技术学院科研基金项目(200509)
关键词
白蒺藜
微波辅助提取
酪氨酸酶
美白剂
正交试验
单因素试验
Tribuius terrestris L.
Microwave- assisted extraction
Tyrosinase
Skin- lightening agent
Orthogonal method
Single factor method
作者简介
龚盛昭（1970-），男，湖南江永，副教授，博士，从事天然产物的开发和应用研究。E—mail：shengzha02002@yahoo．com.cn
