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HPLC-ELSD法测定川牛膝中两种三萜皂苷的含量 被引量:6

Quantitative Analysis of Triterpenoid Saponins in Cyathula officinalis by HPLC-ELSD
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摘要 目的:建立川牛膝中2种主要三萜皂苷——川牛膝皂苷A、B的含量测定方法。方法:采用HPLC-ELSD法,Shim-pack VP-ODS C18(4.6mm×250mm,5μm)色谱柱;流动相为乙腈-0.1%甲酸水(37:63);体积流量为1.0 mL·min^(-1);ELSD漂移管温度为105℃;载气流速2.8 L·min^(-1)。结果:3-O-{β-D-吡喃葡萄糖-(1→2)-[α-L-吡喃鼠李糖-(1→3)]-β-D-吡喃葡萄糖醛酸基}齐墩果酸-28-O-β-D-吡喃葡萄糖苷(川牛膝皂苷A)和3-O-[α-L-吡喃鼠李糖-(1→3)-β-D-吡喃葡萄糖醛酸基]齐墩果酸-28-O-β-D-吡喃葡萄糖苷(川牛膝皂苷B)的含量分别为0.47%~1.37%和0.46%~1.32%;线性范围分别为1.0~20.0μg与0.8~16.0μg;平均回收率分别为99.72%和97.72%。结论:HPLC-ELSD含量测定方法准确、简便、专属性好,适用于川牛膝的质量评价。 To establish a quantitative analysis method of the triterpenoid saponins in Cy-athula officinalis. Methods: An HPLC-ELSD method was used to determine the contents of two major triterpenoid saponins in C. officinalis. The separation was performed on a Shim-pack VP-ODS column (C18, 5μm, 4.6 mm×250 mm), with a mixture solution of acetonitrile-0.1% formic acid in water (37∶63) as mobile phase. The flow rate was 1.0 mL·min-1. The drift tube temperature of ELSD detector was 105℃, the gas flow was 2.8 L·min-1. Results: The contents of 3-O-{β-D-glucopy- ranosyl-(1→2)-[α-L-rhamnopyanosyl-(1→3)]-(β-D-glucuronopyranosyl)}-28-O-β-D-glucopyranosyl oleanolic acid (cyaonoside A) and 3-O-α-L-rhamnopyranosyl-(1→3)- β-D-glucuronopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid (cyaonoside B) were 0.47%-1.37% and 0.46%-1.32%, respectively. The linear range of were 1.0-20.0μg and 0.8-16.0μg and the average recoveries were 99.72% and 97.72%, respectively. Conclusion: The HPLC-ELSD method is accurate and suitable for the quantitative analysis of these two saponins in C. officinalis.
出处 《药学与临床研究》 2014年第4期336-338,共3页 Pharmaceutical and Clinical Research
关键词 川牛膝 三萜皂苷 川牛膝皂苷A B HPLC-ELSD法 含量测定 Cyathula officinalis Triterpenoid saponins Cyanoside A, B HPLC-ELSD method Quantita-tive analysis
作者简介 闫文静.女,硕士生E—mail: 通讯作者;汪豪,男,教授E-mail:wanghao@cpu.edu.cn
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