摘要
目的:研究定痫丸对氯化锂-匹罗卡品致痫小鼠海马组织miRNA-187表达的影响。方法:采用基因芯片技术筛选出氯化锂-匹罗卡品致痫小鼠海马组织差异表达基因,并运用q-PCR技术验证其差异性表达。予定痫丸悬混液灌胃,运用q-PCR技术检测定痫丸悬混液对癫痫小鼠海马组织差异基因的表达。结果:通过基因芯片筛选出癫痫小鼠海马组织中差异表达miRNA下调18个,上调10个。筛选出miRNA-187,运用q-PCR技术验证了其表达下调(P<0.05)。经定痫丸悬混液干预后,发现癫痫小鼠海马组织miRNA-187表达上调(P<0.05)。结论:定痫丸可能通过上调miRNA-187对癫痫起到治疗作用,初步揭示定痫丸治疗癫痫的分子机制。
Objective:To study the effect of Dingxian Pills on the expression of miRNA-187 in hippocampus of LiCl-Pilocarpine-induced epilepsy mice.Methods: The differentially expressed genes in the hippocampus of mice with lithium chloride-pilocarpine-induced epilepsy were screened by gene chip technique and their differential expression was verified by q-PCR.The intervention of Dingxian Pills were intragastrically administered and q-PCR was used to detect the expression of differentially expressed genes in the hippocampus of epileptic mice.Results: Eighteen differentially expressed miRNAs and 10 up-regulated miRNAs were detected in the hippocampus of epileptic mice by microarray.The miRNA-187 was screened and its expression was down-regulated by q-PCR(P<0.05).After intervention of Dingxian Pills suspension, the expression of miRNA-187 in the hippocampus of epileptic mice was found to be upregulated(P<0.05).Conclusion: Dingxian Pills may play a therapeutic role in epilepsy by up-regulating miRNA-187, and initially reveal the molecular mechanism of epilepsy treated by Dingxian Pills.
作者
吴琼
汪顺贵
郭雪峰
李华琼
陈爱玲
刁丽梅
仇小强
WU Qiong;WANG Shungui;GUO Xuefeng;LI Huaqiong;CHEN Ailing;DIAO Limei;QIU Xiaoqiang(The Graduate CoIIege of Guangxi University of Chinese Medicine,Nanning 530000,Guangxi,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,Guangxi,China;The School of Public Health Guangxi Medical University,Nanning 530021,Guangxi,China)
出处
《中华中医药学刊》
CAS
北大核心
2019年第1期116-120,I0007,共6页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81760809)
广西自然科学基金项目(2017GXNSFAA198294)
广西壮族自治区临床重点专科建设项目(桂卫医发[2015]5号文)
广西医疗卫生适宜技术开发与推广应用项目(S2017049)
广西研究生教育创新计划项目(YJSP201710)
作者简介
吴琼(1989-),男,河南淮滨人,硕士研究生,研究方向:中西医结合脑病方向(癫痫、脑血管病等)的临床与基础研究;通讯作者:刁丽梅(1972-),女,山东黄县人,主任医师,硕士研究生导师,博士,研究方向:中西医结合脑病方向(癫痫、脑血管病等)的临床与基础研究。E-mail:dlm721226@163.com;通讯作者:仇小强(1957-),男,广西柳州人,教授,博士研究生导师,博士后指导教师,博士,研究方向:恶性肿瘤、先天畸形及慢性病的临床流行病学、分子流行病学研究。E-mail:xqqiu9999@sina.com。
