摘要
目的:探讨黄芪甲苷(Astragaloside IV)对宫颈癌细胞株Hela的放射敏感性影响并讨论其可能机制。方法:采用MTT法检测黄芪甲苷对Hela细胞生长的抑制作用,克隆形成实验和流式细胞术分别检测Hela细胞的放射敏感性和细胞周期,Tunel法检测Hela细胞凋亡能力,Western blot检测Hela细胞中p-EGFR、EGFR与p-Akt、Akt蛋白表达水平变化。结果:黄芪甲苷抑制Hela细胞的生长,且60μg/mL时抑制作用最大; 60μg/mL黄芪甲苷联合放射治疗后,D_0和D_q值显著小于单独放射时的值,拟合的生存曲线左移,且降低放射诱导的细胞G2/M期阻滞,促进细胞凋亡;黄芪甲苷可显著下调EGFR与Akt的磷酸化水平,EGFR与Akt总蛋白表达水平不变。结论:黄芪甲苷具有增加宫颈癌细胞株Hela放射敏感性的作用,可能与细胞周期G2/M期阻滞和DNA损伤修复蛋白表达水平降低有关。
Objective: To investigate the effects and potential mechanisms of Astragaloside Ⅳ on radiosensitivity of cervical cancer Hela cells. Methods: MTT assay was used to examine the inhibitory effects of Astragaloside IV on Hela cell viability. Radiosensitivity was analyzed by clone formation test,and cell cycle was detected by flow cytometry. The protein expressions of p-EGFR,EGFR,p-Akt and Akt were determined by western blotting. Results: Astragaloside IV decreased the viability of Hela cells. The maximal inhibitory effect was at the concentration of 60 μg/mL. Combination of Astragaloside Ⅳ( 60 μg/mL) and radiotherapy significantly decreased the value of D0 and Dq as compared with radiotherapy alone,and the survival of fitting curve shift to the left. Astragaloside IV inhibited radiation-induced the cell retardation in G2/M phase and promoted apoptosis,and the protein expression of p-EGFR and p-Akt were markedly decreased while the protein expression of EGFR and Akt remained unchanged. Conclusion: Astragaloside IV increases radiosensitivity of Hela cervical cancer cell line through inhibiting cell retardation in G2/M phase and DNA damage-repair protein expression.
作者
李潇
周艳艳
宋晓婕
李岩
邵艳社
LI Xiao;ZHOU Yan-yan;SONG Xiao-jie;LI Yan;SHAO Yan-she(No.2affilated Hospital,Henan University of Chinese Medicine,Zhengzhou,Zhengzhou d50002,China)
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2018年第11期1540-1543,共4页
JOURNAL OF BASIC CHINESE MEDICINE
作者简介
李潇(1980-),女,河南郑州人,主治医师,讲师,医学硕士,从事妇科内分泌疾病的临床与研究;通讯作者:周艳艳,河南郑州人,副主任医师,Tel:13526649210,E-mail:zyy19760803@126.com。
