摘要
目的分析不同生长阶段不同花色(黄花、紫花、深紫花)款冬花中有效成分的积累规律,为款冬花药材生产和质量控制提供理论指导。方法基于HPLC法对不同生长阶段、不同花色款冬花药材进行测定,采用中药色谱指纹图谱相似度评价系统(2012A版)计算相似度,运用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)对样品进行模式识别研究。结果获得了不同生长阶段和不同花色的款冬花药材HPLC指纹图谱,标定了27个共有峰,指认了其中11个共有峰;30批样品与对照指纹图谱的相似度极高,在0.901～0.995;根据峰面积可以判断出款冬花药材在不同生长阶段其积累特征存在差异,不同花色间差异明显;通过PCA和PLS-DA可将不同花色款冬花样品完全区分开来,4号峰没食子酸、6号峰绿原酸、8号峰芦丁、9号峰金丝桃苷、11号峰异绿原酸B、17号峰槲皮素等12个成分是导致不同花色款冬花质量差异性的主要标志物。质量评价以深紫花质量最好,紫花次之,黄花最差。结论 HPLC指纹图谱能够反映款冬花有效成分在不同生长阶段的积累特征和不同花色间的差异,结合化学模式识别,可为款冬花药材的生产和质量评价提供参考。
Objective To analyze the accumulation patterns of active constituents in Farfarae Flos with different flower bud colors(yellow,purple,and deep purple) at different growth stages,and provide theoretical guidance for the production and quality control of Farfarae Flos.Methods The medicinal materials of Farfarae Flos of different growth stages with different colors were determinated by HPLC method.The Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 A edition) was used to evaluate the similarity of the samples.The differences among samples were identified by chemical pattern recognition methods including hierarchical principal component analysis(PCA) and partial least squares discriminate analysis(PLS-DA).Results The HPLC fingerprint of different flower bud colors of Farfarae Flos at different growth stages was obtained,27 common peaks were found in the chromatography,and 11 of them were identified.Similarities of samples of all batches with reference fingerprint were among 0.901—0.995.There were differences in the accumulation characteristics of Farfarae Flos at different growth stages according to the peak area,and showing significant differences among different flower bud colors.PCA and PLS-DA results demonstrated obvious distinction among different flower bud colors.Twelve constituents,such as gallic acid,chlorogenic acid,rutin,hyperin,isochlorogenic acid B and quercetin,were screened as biomarkers,representing major differences among colors.The quality evaluation demonstrated that deep purple buds was the best,followed by purple buds and yellow buds for the worst.Conclusion The HPLC fingerprint can reflect the accumulation characteristics of the active constituents of Farfarae Flos in different growth stages and the differences among different flower bud colors.Combining chemical pattern recognition can provide reference for the production and quality evaluation of Farfarae Flos.
作者
彭亮
杨冰月
张琳
颜永刚
陈国栋
陈国良
赵璠
胡本祥
PENG Liang;YANG Bing-yue;ZHANG Lin;YAN Yong-gang;CHEN Guo-dong;CHEN Guo-liang;ZHAO Fan;HU Ben-xiang(College of Pharmacy,Shaanxi University of Chinese Medicine,Xi'an 712046,China;Shaanxi Guangjitang Pharmaceutical Group,Yulin 719000,China;Yulin Hospital of Traditional Chinese Medicine,Yulin 719000,China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第21期5018-5023,共6页
Chinese Traditional and Herbal Drugs
基金
国家中药标准化项目(ZYBZH-Y-QIN-36:202410001)
公益性行业(中医药)科研专项经费项目(201507002-1-08)
陕西省高校青年杰出人才支持计划项目
咸阳市中青年科技领军人才项目
作者简介
彭亮(1985-),博士,研究方向为中药资源评价与开发利用、中药材质量控制研究。E-mail:ppengliang@126.com;通信作者:杨冰月(1983-),博士,研究方向为中药品种、质量及资源开发研究。E-mail:304951774@qq.com;通信作者:胡本祥(1960-),教授,研究方向为中药品种、质量及资源开发研究。E-mail:hubenxiang@tom.com
