摘要
目的：优化盐泽泻的炮制工艺,明确其工艺参数,为盐泽泻的规范化生产和质量控制提供实验依据。方法：以环氧泽泻烯,23-乙酰泽泻醇C,泽泻烯醇,泽泻醇B和23-乙酰泽泻醇B成分总量、醇溶性浸出物质量分数和外观性状评分的综合评分为指标,采用单因素试验和正交试验考察闷润温度、闷润时间、切片厚度和干燥温度及盐炙工艺中的盐浓度、盐水闷润时间、溶液用量、炒制温度和炒制时间对盐泽泻炮制工艺的影响。采用UPLC测定5个指标成分的含量,流动相乙腈-水梯度洗脱,检测波长208 nm。结果：盐泽泻最佳炮制工艺为泽泻药材75℃闷润1.0 h后趁热切4 mm厚片,65℃干燥得到泽泻饮片,每100 kg泽泻饮片加盐水13 kg（溶解了2.0 kg盐）,闷润3 h,在160℃下炒制8 min。结论：优化的盐泽泻中试生产工艺具有较好的可行性和可控性,适用于盐泽泻的工业化生产。
Objective： To optimize the processing technology of Alismatis Rhizoma with salt,and clarify the processing parameters,in order to provide the basis for standardized production and quality control of Alismatis Rhizoma. Method： With comprehensive score of total content of five ingredients（ alismoxide,23-acetyl alisol C,alisenol,alisol B and 23-acetyl alisol B）,content of alcohol soluble extract and appearance score as indicator,single factor tests and orthogonal test were adopted to optimize processing technology of salt-processed Alismatis Rhizoma by taking moistening temperature, moistening time, slice thickness and others as factors. UPLC was employed to determine contents of these five ingredients with mobile phase of acetonitrile-water for gradient elution and detection wavelength at 208 nm. Result： Optimum processing technology of salt-processed Alismatis Rhizoma was as followings： moistening temperature of 75 ℃,slice thicknesses of 4 mm,moistening time of 1. 0 h,drying temperature of 65 ℃,2 kg salt dissolved with 13 kg water for each 100 kg of Alismatis Rhizoma,moistening for3 h,stir-frying for 8 minutes under 160 ℃. Conclusion： This optimized production process of salt-processed Alismatis Rhizoma has good feasibility and controllability, which is suitable for industrial production of this decoction pieces.
作者
邰艳妮
樊李明
陈秀霞
褚克丹
林青青
金琦
冯琳雪
谢瑞华
TAI Yan-ni;FAN Li-ming;CHEN Xiu-xia;CHU Ke-dan;LIN Qing-qing;JIN Qi;FENG Lin-xue;XIE Rui-hua(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Fujian Chengtian Pharmaceutical Co.Ltd.,Nanping 354099,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第23期37-45,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中药标准化项目(ZYBZH-Y-FJ-09)
福建省中药原料质量监测技术服务中心(财社[2014]76号)
关键词
盐泽泻
生产工艺
三萜类
正交试验
倍半萜类
泽泻
标准化
salt-processed Alismatis Rhizoma
production process
triterpenoids
orthogonal test
sesquiterpenoids
Alismatis Rhizoma
standardization
作者简介
[第一作者]邰艳妮，在读硕士，从事中药质量标准研究，E-mail：1151545049@qq．com;[通信作者]褚克丹，博士生导师，教授，从事中药制剂及质量控制研究，Tel：0591-22861661，E-mail：chukd5917@163．com;[通信作者]林青青，博士，讲师，从事中药鉴定及中药资源学研究，E-mail：542823860@qq．com
