摘要
目的建立藿香正气滴丸的指纹图谱分析方法，更全面分析产品质量。方法运用超高效液相色谱仪的多波长切换技术。色谱条件：三键C18烷基键合技术的ACQUITY UPLC—HSS T3（2．1＊100mm，1．7μm）微径色谱柱，以乙腈-0．5％冰醋酸水为流动相，梯度洗脱；检测波长010min：294nm、10—24min：254nm、24-32min：336nm；柱温：30℃；进样量：1μL。结果运用多波长切换技术所得指纹图谱方法学考察结果良好，采用色谱指纹图谱相似度评价软件，对藿香正气滴丸多波长切换指纹图谱进行相似度计算，其10批样品相似度均〉O．99，说明多批次样品具有代表性。结论该方法快速准确，能全面体现特征吸收波长相差较大的多个成分的指纹信息，能够为藿香正气滴丸的质量控制提供参考。
Objective To establish a method for Huoxiang Zhengqi Dripping Pills fingerprint detection by the multi-wavelength switching technique in order to acquire more information about Huoxiang Zhengqi Dripping Pills. Methods UPLC was used to establish fingerprints with ACQUITY UPLC-HSS T3 （2.1＊ 100mm, 1.7 μm） column and acetonitrile-0.5% glacial acetic acid aqueous, a gradient eluting agent. Wavelength：0-10min：294nm; 10-24min：254nm;24-32min：336nm.Column temperature： 30℃; sample size： 1μL.Results Methodological study showed multi -wavelength switching technique could fully reflect the fingerprint characteristics which components has different absorption wavelength. Using chromatographic fingerprint evaluating software, calculate Huoxiang Zhengqi Dripping Pills multi -wavelength switching fingerprints similarity, the 10 batches of samples similarity all above 0.99. Conclusion The method was sensitive simple and accurate, Multi-wavelength switching enforces the overall evaluation of Huoxiang Zhengqi Dripping Pills and provides a suggestion for enhancing the Huoxiang Zhengqi Dripping Pills quality control.
出处
《现代中药研究与实践》
CAS
2014年第2期55-57,共3页
Research and Practice on Chinese Medicines
关键词
藿香正气滴丸
多波长切换
指纹图谱
Huoxiang Zhengqi Dripping Pills
multi-wavelength switching technique
fingerprint
作者简介
作者简介：阚红玉（1982-），女，硕士研究生，工程师。E—mail：khy820527@sina．com，Tel：022—86342535
