摘要
目的建立广藿香药材不同部位的UPLC指纹图谱,研究广藿香不同部位的质量差异。方法采用UPLC法建立广藿香不同部位化学指纹图谱,结合相似度评价、热图聚类分析(CA)、主成分分析(PCA)及正交偏最小二乘法-判别分析(OPLS-DA)对18批广藿香药材、茎、叶的UPLC指纹图谱进行分析,并测定广藿香酮含量。结果18批广藿香药材及不同部位的UPLC指纹图谱均确定了9个相同的共有峰,并通过对照品指认4、6、9号峰分别为毛蕊花糖苷、异毛蕊花糖苷和广藿香酮。CA和PCA结果表明广藿香叶和茎的质量差异大,叶和药材的质量较接近。OPLS-DA发现5种成分是造成不同批次样品质量差异的主要标志物。含量测定结果表明同一批广藿香中的广藿香酮含量均为茎>药材>叶。结论广藿香不同部位的化学成分相似,但含量存在显著性差异,本研究可为广藿香药材的质量控制及资源开发利用提供参考。
Objective To establish the UPLC fingerprints of different parts of pogostemonis herba,and to study the quality difference of different parts of pogostemonis herba.MethodsThe chemical fingerprints of different parts of pogostemonis herba were established by UPLC method,and the UPLC fingerprints of 18 batches of patchouli medicinal materials,stems and leaves were analyzed by similarity evaluation,heat map cluster analysis(CA),principal component analysis(PCA)and orthogonal least squares discriminant analysis(OPLS-DA),and the patchoulone content was determined.Results The UPLC fingerprints of 18 batches of pogostemonis herba medicinal materials and different parts identified 9 identical peaks,and the 4th,6th,and 9th peaks were identified as verbascoside,isoverbasin and patchoulone,respectively,by the reference substance.The results of CA and PCA showed that the quality of pogostemonis herba leaves and stems were different,and the quality of leaves and medicinal materials were similar.OPLS-DA found that 5 components were the main markers for the quality differences of different batches of samples.The content determination results showed that the content of patchoulone in the same batch of patchouli was all stem>medicine>leaf.Conclusion The chemical components of different parts of pogostemonis herba are similar,but there are significant differences in content.This study can provide a reference for the quality control and resource development and utilization of pogostemonis herba.
作者
邓桂海
甘力帆
施文婷
张兰兰
胡懿
黎桃敏
张正
魏梅
Gui-Hai DENG;Li-Fan GAN;Wen-Ting SHI;Lan-Lan ZHANG;Yi HU;Tao-Min LI;Zheng ZHANG;Mei WEI(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co.,Ltd.Foshan 528244,Guangdong Province,China)
出处
《药物流行病学杂志》
CAS
2023年第5期528-537,共10页
Chinese Journal of Pharmacoepidemiology
关键词
广藿香
UPLC指纹图谱
化学模式识别
广藿香酮
Pogostemonis herba
UPLC fingerprint
Chemical pattern recognition
Patchoulone
作者简介
通信作者:张正,硕士,主管中药师,Email:524928277@qq.com
