摘要
目的：建立黄芪皂苷类成分注射液（包括药材、中间体及注射液制剂）的HPIE-ELSD指纹图谱质量控制方法。方法：Agilent Zorbax SB-C18柱，流动相为A水-B乙腈，二元梯度洗脱：15％～60％B（0—65min），流速0．8mL·mi^-1，蒸发光散射检测器，漂移管温度105℃，载气流速2．7L·min^-1。结果：药材、中间体、注射液制剂中标定的各共有峰相对保留时间的RSD值均小于1．0％，单峰面积大于或等于10％峰的峰面积比值也比较稳定，三者的图谱间具有很好的相关性，10批样品间具有很高的相似度。结论：本方法可作为黄芪皂苷注射液质量控制的依据。
Objective： To develop an HPLC method for fingerprint determination of the astragalosides injection. Method： Analyses were carried out at 25℃ on a Zorbax SB-C18 column （4.6 mm× 250 mm,5 μm）. Mobile phase A was water, and B was acetonitrile. The analysis followed a linear gradient program. Initial conditions were 15% B; 0 ～ 65 rain, changed to 60% B. The flow rate was 0.8 mL·min^-1 The drift tube temparature of the ELSD was 105℃, and gas flowrate was 2.7 L·min^-1. Result： The RSD of relative retention time of the common peaks in the plant material, the extract and the injection fingerprints was less than 0. 1%, and there was good similarity among 10 batches of samples. Condusion： The method was reliable and simple which could be used for quality control of the injection.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第10期807-809,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重大资助项目(90209045)
关键词
指纹图谱
黄芪皂苷
注射液
HPLG fingerprint
astragaloside
injection
作者简介
[通讯作者]李萍．Tel：（025）85322256，E-mail：lipingli@publicl．ptt．js.cn
