摘要
目的:分析比较枳实、枳壳中辛弗林和挥发油含量,探讨两者功效差异的原因。方法:采用反相离子对高效液相色谱(HPLC)法测定辛弗林含量,色谱柱:Diamonsil C18(5μm,200mm×4.6mm),流动相:甲醇-磷酸二氢钾溶液(取磷酸二氢钾0.6 g,十二烷基磺酸钠1.0 g,冰醋酸1 mL,加水溶解并稀释至1000 mL)(50∶50),流速:1 mL·min-1,柱温:25℃,检测波长为275nm。以《中华人民共和国药典》法测定挥发油含量。结果:枳实中辛弗林含量明显高于枳壳,而挥发油含量则是枳壳高于枳实。结论:枳实和枳壳药材有效成分(辛弗林和挥发油)的含量存在明显差异,是导致其功效差异的原因之一,临床处方应区别应用。
Objective ： To analyze the contents of syperphrine and volatile oil in Aurantii fructus immaturus and Aurantii fructus. Methods ： Reversed phase ion - pair HPLC method was used for the contents determination of synephrine. It was performed on Diamonsil C I8 （51.Lm,200 mm x 4.6 mm）. The mobile phase was CH3 CN- KHzPO4 （50：50）（0.6 g KH2 PO4, 1.0 g sodium dodecyl sulfate and 1 mL glacial aceticacid were dissolved and diluted to 1000 mL with water） ,with a flow rate of 1 mL ~ min-1. Column temperature was 25% and wavelength was 275 nm. The contents of volatile oil were determined with the method of Chinese Pharmacopeia. Results： Contents of syperphrine in Aurantii fructus immaturus were higher than that in Aurantii fructus,but the results of volatile oil were opposite to that of syperphrine. Conclusion： The contents of syperphrine and volatile oil vary significantly in Aurantii fructus immaturus and Aurantii fructus, which may be one of the possible causes of their different efficacy.
出处
《中华中医药学刊》
CAS
2014年第3期665-667,共3页
Chinese Archives of Traditional Chinese Medicine
关键词
枳实
枳壳
反相离子对高效液相色谱
辛弗林
挥发油
功效
Aurantii fructus immaturus
Aurantii fructus
HPLC
syperphrine
volatile oil
efficacy
作者简介
吴学谨（1973-），女，浙江桐庐人，主管中药师，研究方向：中药鉴定、质量分析和临床应用。
通讯作者：陈建真（1965-），女，浙江杭州人，教授，博士，研究方向：中药有效成分和质量控制研究。E-mail：chjzl02@yahoo．com．cn
