摘要
目的建立同时测定桑叶中绿原酸、芦丁和木犀草苷含量的方法。方法样品用70％乙醇溶液提取，采用Phenomenex LunaC18色谱柱（4．6mm×250mm，5μm）分离；流动相：0．1％磷酸-乙腈，梯度洗脱；流速：1：0mL·min^-1；检测波长：348nm。结果绿原酸、芦丁、木犀草苷分别在3．048～152．4，1．059～52．96，1．437-71．85μg·mL一内具有良好的线性关系；加标回收率分别为100．4％，98.3％，98．7％；RSD分别为1．7％，2．0％，1．9％（n=9）。结论该方法简单易行，准确性和重复性好，可用于桑叶的质量控制。
OBJECTIVE To determine the content of chlorogenic acid, rutin and luteolin glycosides in mulberry leaves by HPLC. METHODS The sample was extracted by 70% ethanol and separated on a Phenomenex Luna column（4.6 mm × 250 mm, 5 μm）. 0.1% Phosphoric acid and acetonitrile was used as mobile phase with gradient elntion at flow rate of 1.0 mL.min^-1 The detection wavelength was set at 348 nm. RESULTS The linear ranges of chlorogenic acid, rntin and luteolin glycosides were 3.048-152.4, 1.059-52.96 and 1.437-71.85 μg.mL^-1. And the average recoveries were 100.4%, 98.3% and 98.7% with the RSD of 1.7%, 2.0%, 1.9%（n=9）, respectively. CONCLUSION This method is simple, rapid and with good repeatability, and can be used for the quality control of mulberry leaves.
出处
《中国现代应用药学》
CAS
CSCD
2014年第2期218-221,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
高效液相色谱法
桑叶
绿原酸
芦丁
木犀草苷
HPLC
mulberry leaves
chlorogenic acid
rutin
luteolin glycosides
作者简介
郑娟，女，主管中药师Tel：（0571）85463891E—mail：zhengjuan0@hz．cn
