摘要
目的:建立测定钩藤中7种生物碱类成分含量的一测多评方法,探讨其可行性。方法:以钩藤碱为内参物,建立去氢钩藤碱、异钩藤碱、异去氢钩藤碱、缝籽嗪甲醚、去氢毛钩藤碱、毛钩藤碱6种生物碱类成分的相对校正因子;采用外标法验证一测多评法的可行性。结果:建立了一测多评法同时测定钩藤中7种成分的含量,去氢钩藤碱、异钩藤碱、异去氢钩藤碱、缝籽嗪甲醚、去氢毛钩藤碱、毛钩藤碱的相对校正因子分别为0.959、1.002、0.973、0.663、0.658和0.790。外标法与一测多评法定量结果无显著差异。结论:建立的一测多评法快速、准确、耐用性好,可用于该品种钩藤中7种生物碱类成分的定量分析,为建立简便、可靠的钩藤质量控制方法提供了基础。
Objective:To establish a QAMS method for the content determination of seven alkaloids in Uncaria rhynchophylla and to validate its feasibilities.Methods:Rhynchophylline was used as the internal reference substance,the relative correlation factors of six alkaloids(corynoxeine,isorhynchophylline,isocorynoxeine,geissoschizine methyl ether,hirsuteine,hirsutine)were calculated and evaluated.The feasibility of QAMS method was verified by the external standard method.Results:The QAMS method was established and the relative correction factors of corynoxeine,isorhynchophylline,isocorynoxeine,geissoschizine methyl ether,hirsuteine and hirsutine were 0.959,1.002,0.973,0.663,0.658 and 0.790,respectively.There was no significant difference between the QAMS method and external standard method.Conclusion:The established QAMS method is fast,accurate and durable,and can be used for quantitative analysis of seven alkaloids in Uncaria rhynchophylla,which provides a basis for establishing a simple and reliable method for quality control of Uncaria rhynchophylla.
作者
梁颖欣
朱旦
黄舒婷
曾常青
杨仟
LIANG Ying-xin;ZHU Dan;HUANG Shu-ting;ZENG Chang-qing;YANG Qian(College of Traditional Chinese Medicine,Guangdong Pharmaceutical University/State Key Laboratory of Digital Quality Evaluation Technology for Traditional Chinese Medicine,State Administration of Traditional Chinese Medicine,Guangzhou 510006,China;Uncaria Institute,Jianhe County Science and Technology Bureau,Guizhou Province,Jianhe 556400,China)
出处
《中药材》
CAS
北大核心
2019年第8期1820-1823,共4页
Journal of Chinese Medicinal Materials
基金
贵州省剑河县中药材产业科技合作专项计划(剑科合字[2014]4号).
关键词
钩藤
一测多评
相对校正因子
Uncaria rhynchophylla(Miq.)Miq.ex Havil.
QAMS
Relative correction factor
作者简介
梁颖欣(1994-),女,在读硕士研究生,专业方向:中药质量分析与评价,Tel:13422294499,E-mail:783530092@qq.com;通讯作者:曾常青,Tel:13527729559,E-mail:gdzcq@163.com。
