摘要
以纤维堆囊菌SOF5-76为试验菌株,用响应面分析法对其产埃博霉素B的培养基进行优化,以提高埃博霉素B的产量。在单因素试验的基础上,利用Plackett-Burman筛选出对埃博霉素B产量有显著影响的3个因素为马铃薯淀粉、脱脂奶粉和无水氯化钙,在此基础上通过最陡爬坡试验逼近最佳响应面区域;再运用Box-Behnken试验设计和响应面分析法进行回归分析,确定重要因素的最优浓度。得到最佳发酵培养基为:马铃薯淀粉3.9 g/L、脱脂奶粉2.2 g/L、无水氯化钙1.3 g/L、葡萄糖1 g/L,豆饼粉1.5g/L,七水硫酸镁2.5 g/L,EDTA-Fe3+3 mL/L,微量元素(TE)0.5 mL/L,VB121 mL/L。在此最优条件下发酵埃博霉素B的产量为29.95 mg/L,与模型预测值接近,发酵产量比优化前提高了1.1倍。
The response surface methodology （ RSM ） was employed to optimize the medium composition for Epothilone B produced by Sorangium cellulosum SOF5-76. On the basis of one-variable-at-a-time design, using Plackett-Burrnan design, potato starch, skimmed milk powder and calcium chloride were screened out of 9 factors as main affecting variables of Epothilone B production, and then steepest ascent method was used to approach their maximum response regions, followed by Box-Behnken design, multiple regression analysis and response surface analysis. The optimum medium formula determined was composed of potato starch 3.9 g/L, skim milk powder 2.2 g/L, chlorine calcium 1.3 g/L, glucose 1 g/L, soybean powder 1.5 g/L, magnesium sulfate 2.5 g/L, EDTA-Fe3＋ 3 mL/L, trace elements 0.5 mL/L, VBt21 mL/L. Under this optimal conditions, Epothilone B production reached up to 29.95 mg/L and increased 1.1 times in average as compared with preliminary culture and consistent with the maxmum predicted value.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第1期171-176,共6页
Biotechnology Bulletin
基金
陕西省教育厅自然科学专项(12JK1023)
国家自然科学基金项目(20906058)
关键词
埃博霉素B纤维堆囊菌发酵培养基响应面法优化
Epothilone B Sorangium cellulosum Fermentation medium Response surface methodology optimization
作者简介
龚国利，男，副教授，硕士生导师，研究方向：生物制药技术；E-mail：gongguoli@sust．edu.cn
