摘要
埃博霉素发酵生产体系复杂,且生产菌株较难操作,导致发酵参数测定困难,迄今为止未见关于埃博霉素发酵动力学方面的报道。利用5 L发酵罐,对纤维堆囊菌(Sorangium cellulosum)产埃博霉素B(Epothilone B)的分批发酵动力学进行了研究,整个发酵过程中维持温度30℃、p H7.4。发酵结束时,菌体干重达3.00 g/L,埃博霉素B产量达18.20 mg/L,葡萄糖含量为0.049g/L。基于Logistic方程和Luedking-Piret方程,利用MATLAB软件对其进行非线性拟合,构建了菌体生长、埃博霉素B合成和葡萄糖消耗的动力学模型。结果表明,该组模型能较好的拟合发酵过程。
The fermentation system produced epothilone is complex, and the production strain is more difficult to operate, leading to difficulties in fermentation parameters measured, so far there is no reporting on Epothilone fermentation kinetics. In this paper, the batch fermentation kinetics of the epothilone B produced by Sorangium cellulosum SoF5-76 was studied in a 5 L fermentor. During the process, the temperature and pH were almost constant, 30℃, 7.4. When the fermentation finished, the dry cell weight, epothilone B production and residual glycerol could reach 3.00 g/L, 18.20 mg/L and 0.049 g/L. On the bases of Logistic and Luedking-Piret equation, the models of cell growth, epothilone B production and substrate consumption were established, and the non-line fitting was employed to model the kinetics of fermentation by software MATLAB. The results show that the kinetic models were in good agreement with the fermentation process.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第2期202-207,共6页
Biotechnology Bulletin
基金
国家自然科学基金项目(20906058)
陕西科技大学学术骨干培育项目(XSG2010009)
作者简介
马利云，男，硕士，研究方向：微生物制药；E-mail：mayun919585594@163．com
通讯作者：龚国利，男，博士，教授，研究方向：工业微生物发酵工程技术；E-mail：gongguoli@sust．edu．cn
