摘要
在单因素实验基础上,利用响应面法对影响超声辅助提取田基黄总多酚的主要因素(乙醇浓度、料液比、超声温度和时间)进行了优化,建立了影响因素与总多酚之间的函数关系。获得最佳工艺条件为70%乙醇,料液比1∶25(g/mL),超声提取温度46℃,提取时间50 min。在此最佳条件下,总多酚提取率为1.3189%,试验结果与模型预测值相符。
Response surface methodology （RSM） was used to optimize ultrasonic-assisted extraction of total polyphenols from Hypericum japonicum based on single-factor experiments. The main influencing factors, including ethanol concentra- tion, materiai-solvent ratio,extraction temperature and time were determined. The function relationship between influen- cing factors and response values was established. The optimum extraction conditions were as follows：the extraction sol- vent was 70% ethanol,material-solvent ratio was 1：25 （g/mL）, extraction temperature was 46 ℃, and extraction time was 50 min. Under these conditions,the extraction rate of total polyphenols reached 1. 3189% ,which was well matched with the predicted value.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第12期1715-1721,共7页
Natural Product Research and Development
基金
湖南省科技厅科技计划项目(2008FJ3077)
关键词
田基黄
总多酚
响应面法
Hypericum japonicum
total polyphenol
response surface methodology
作者简介
通讯作者Tel：86-743-8563911：E-mail：ouyang1227@126．com
