摘要
目的建立测定生新发胶囊中特女贞苷含量的方法。方法采用高效液相色谱法，色谱柱为hgilentTC—C18（4．6mm×250mm，5μm），流动相为甲醇-水（35：65），流速为1．0mL／min，检测波长为224nm。结果特女贞苷线性范围为0．15—0．90μg，平均加样回收率为l00．35％，RSD=1．43％。结论本方法准确、可靠、重复性好，能测定生新发胶囊中特女贞苷的含量，可作为生新发胶囊质量控制的含量检测方法。
Objective To set up a method for determining Specnuezhenide in Shengxinfa Capsules. Methods With HPLC method, Agilent TC-C18 column （4.6 min×250 mm, 5 μm） was used. Mobile phase was methanol-water （35 ： 65）, the flow rate was 1.0 mL/min, and the detecting wavelength was at 224 nm. Results The linearity was in the range of 0.15-0.90μg. The average recovery was 100.35%, and RSD was 1.43%. Conclusion The method is accurate, reliable, and can be used for the determination of Specnuezhenide in Shengxinfa Caosules for its aualitv control.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第12期54-55,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
广西壮族自治区科技攻关项目(桂科攻1099062-11)
