摘要
建立了同时测定墨旱莲中香豆草醚类成分蟛蜞菊内酯和异去甲基蟛蜞菊内酯含量的高效液相色谱法。采用Kromasil C18柱（250mm×4．6mm，5um），流动相为甲醇-0．5％醋酸水溶液（体积比为55：45），流速为1．0mL／min，检测波长为351nm，柱温为30℃，进样量为20uL。在上述条件下测得的异去甲基蟛蜞菊内酯和蟛蜞菊内酯的质量浓度分别在1．6～32．0mg／L和5．6～112．0mg／L时与色谱峰面积之间的线性关系良好，二者高、中、低浓度条件下的平均加标回收率分别为97．5％～98．2％和99．0％～100．2％。该方法简便、快速、准确，可作为墨旱莲质量控制的一个有效方法。
A reversed-phase high performance liquid chromatographic method （RP-HPLC） was developed for the simultaneous determination of wedelolactone and isodemethylwedelolactone in Herba Ecliptae from different sources. The separation was performed on a Kromasil C18 column （250 mm × 4. 6 mm, 5 um）, with methanol-0.5% acetic acid （ 55 ： 45, v/v） as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 351 nm and the volume of injection was 20 uL. There were good linear relationships between the mass concentrations and the peak areas of isodemethylwedelolactone and wedelolactone in the ranges of 1.6 - 32.0 mg/L （r =0.9995） and 5.6 - 112.0 mg/L （r =0.9998）, respectively. The recoveries were found to be in the range of 97.5% - 98.2% and 99.0% - 100. 296, respectively. The results of the experiments have demonstrated that the established method is rapid and simple with good accuracy and reproducibility for the simultaneous determination of wedelolactone and isodemethylwedelolactone. The method is suitable for the quality control of Herba Ecliptae from different sources.
出处
《色谱》
CAS
CSCD
北大核心
2007年第3期371-373,共3页
Chinese Journal of Chromatography
关键词
高效液相色谱法
蟛蜞菊内酯
异去甲基蟛蜞菊内酯
墨旱莲
reversed-phase high performance liquid chromatography （RP-HPLC）
wedelolactone
isodemethylwedelolactone
Herba Ecliptae
作者简介
原红霞，女，硕士研究生，Tel：（024）82690970，E-mail：yuanhongxia609@163.com
通讯联系人：于治国，男，副教授，Tel：（024）23986295，E-mail：zhiguo-yu@163.com.
