摘要
为建立一种香蕉中壬基酚残留的提取和检测方法,样品经乙腈提取,C18固相萃取柱净化浓缩后,洗脱液用高效液相色谱检测。在优化条件下,壬基酚在0.05～5mg·L-1的范围内线性关系良好,相关系数达0.999 9,仪器最低检测浓度为2μg·kg-1。样品中添加3个水平的壬基酚,5次重复测定的平均回收率为78.1%～93.4%,相对标准偏差为3.71%～5.38%,符合残留分析的要求。
The extraction and detection method of nonylphenol residue in banana were establishmed. Samples were extracted with acetonhrile, purified and concentrated by C18 solid phase extraction column, and the eluent was determined by high performance liquid chromatography. Under the optimized conditions, nonylphenol in 0.05-5 m·L^-1 range had a good linear relationship, the correlation coefficient reached 0. 999 9, the detection limit was 2 μg·kg^-1. The average recoveries for nonylphenol at 3 spiked levels were 78. 1% -93. 4% and the RSD were 3.71%- 5. 38% for 5 repeatative determinations, the results meet the requirement of residue analysis.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2013年第3期86-89,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家高技术研究发展计划项目(863-2012AA03A607)
江苏省农业委员会科研项目(JSNZJ2011006)
关键词
香蕉
壬基酚
高效液相色谱
固相萃取
检测
banana
nonyphenol
high performance liquid chromatography
solid-phase extraction
determination
作者简介
葛洪（1962-），男，江苏扬州人，扬州环境资源职业技术学院副教授，主要从事农产品质量安全检测研究。E—mail：hang_g1106@sohu．com
