摘要
采用OPA柱前衍生氨基酸、多步梯度洗脱、荧光检测器检测、高效液相色谱法，在45分钟内分离出16种氨基酸。该法样品用量少，灵敏度高，线性范围宽，重视性好，适于分析大量样品。
The authors pasent a method for detendning the awhno adds in urine samples by HPLC with fluorescence detector. More than 16 amin acid were separated within 45 min by using pre-colunm derivathation with O-phthalaklehyde and a multi-step grudot with two solvents. The method is sensitive, reprodudble, accurate and has a lincar rang from 125 -400 pmol.
出处
《黑龙江大学自然科学学报》
CAS
1997年第2期100-102,共3页
Journal of Natural Science of Heilongjiang University
关键词
高效液相色谱
氨基酸
荧光检测器
High performance liquid chromatography
Amino acid, Fluorescence detector
