摘要
采用平板溶磷圈法对典型黑土区4种林地（美青杨林、水曲柳林、落叶松林和樟子松林）土壤的磷细茵进行了分离和筛选．并测定了其解磷能力。结果表明：4种林地土壤中分布了大量的解磷菌和溶磷茵，其中每克解磷茵的茵落形成单位（CFU）为（5．43～16．81）×10^5、溶磷菌为（0．49-3．16）×10^5，且解磷菌数量多于溶磷菌。从4种林地土壤中分离得到了15株分解有机磷的解磷菌和11株溶解无机磷的溶磷茵；其中有2株解磷茵解磷能力较高，解磷圈直径与茵落直径比值均大于2．0，解磷量分别为0．50、1．13mg／L；有2株溶磷茵溶磷能力较高，溶磷圈直径与菌落直径比值均大于2．0，溶磷量分别为0．90、0．18mg／L。
The experiment was conducted to study the phosphorn bacterial strains from four kinds of forests soil including Poplar （Populus nigra var. italicaxP, cathayan ） , Manchurian Ash （Fraxinus mandshurica ） , Larch （Larix gmelini ） and Scots Pine （ Pinus sylvestris var. mongolica） in typical black soil region of northeastern China by plate P-solubilizing circle meth- od. The vast amounts of both phosphate-dissolving and phosphate-solubilizaing bacteria was distributed from four kinds of forest soils, which are in the ranges of （5.43-16.81 ）×10^5 cfu ·g-1 and （0.49-3.16）×10^5 cfu·g-l, respectively. 15 phosphate-dissolving bacterial strains and 11 phosphate-solubilizing bacterial strains with obvious dissolving phosphate zone were obtained from four kinds of forests soil. Two strains show strong ability of dissolving phosphate with a ratio of dissol- ving phosphate zone diameter to culture community zone diameter （D/d） exceeding 2.0, and phosphate-dissolving capaci- ty were O. 50 and 1.13 mg/L, respectively. Two phosphate-solubilizing bacterial strains with stronger phosphate-solubiliz- ing capability were obtained with D/d of more than 2.0, and the phosphate-solubilizing capacity were 0.90 and 0.18 mg/ L, respectively.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2013年第11期83-85,122,共4页
Journal of Northeast Forestry University
基金
大学生创新实验项目(201210225088)
关键词
解磷细菌
解磷能力
溶磷圈
黑土
Phosphate-solubilizing bacteria
Phosphate-solubilizing capability
Dissolving phosphate zone
Black soil
作者简介
刘聪，女，1990年3月生，东北林业大学林学院，在读学士。
通信作者：陈祥伟，东北林业大学林学院，教授。E-mail：ehenx．wnefu@163．com。
