摘要
为了获得具有高效解磷解钾能力、适宜作为菌肥生产菌的根际促生菌,采用选择性培养基,以稀释培养法针对性地筛选具有解无机磷、解钾能力的根际促生菌,通过生理生化实验结合16S rDNA序列分析对分离菌株进行鉴定,并比较其解磷解钾效果,筛选出无拮抗的菌株并发酵菌液作为菌肥应用。结果表明:多个细菌菌株中,b6巨大芽孢杆菌(Bacillus megaterium)解无机磷活性较强,b7胶质芽孢杆菌(Bacillus mucitaginosus)解钾活性较强。复合菌肥在实验室盆栽条件下对山毛豆(Tephrosia candida)促生效果显著,但其肥效与化肥仍有差距,可搭配减量化肥施用,提高化肥利用率。
In order to obtain plant growth-promoting rhizobacteria （ PGPR ） strains with efficient activity on solubilizing phosphate or potassium, and the potentiality as fertilizer-producing bacteria, strains were isolated by using selective medium and diluting culture, then they were identified after a series of physiological and biochemical experiments and 16S rDNA sequence analysis. And the solubilization activity was tested, unopposed strains were selected and used as biological bacterial fertilizer. The results showed that b6 （ Bacillus megaterium ） had efficient activity on solubilizing inorganic phosphorus, b7 （ B. mucitaginosus ） had efficient activity on solubilizing potassium. Under laboratory conditions, the compound biological fertilizer had a significant promoting effect on Tephrosia candida, but it still couldn＇ t replace chemical fertilizers. Biological bacterial fertilizer can be used with the reduction of chemical fertilizer, to improve the fertilizer utilization.
出处
《广东农业科学》
CAS
2017年第3期94-100,共7页
Guangdong Agricultural Sciences
基金
广东省科技计划项目(2014B090903015
2015B090904008)
关键词
根际促生菌
解钾
解无机磷
鉴定
筛选
plant growth-promoting rhizobacteria （ PGPR ）
potassium-solubilizing
inorganic phosphorus-solubilizing
identification
screening and application
作者简介
宋凤鸣（1987-），女，硕士，助理工程师，E—mail：490257804@qq．com
通讯作者：许建新（1982-），男，博士，高级工程师，E—mail：582546461@qq．com
