摘要
以葛根粗提物为原料,比较D101、X-5、HD-101、D14、S-8、HC-D301、HD-8、D141、HPD100、HPD500、AB-8及NKA-2共12种大孔吸附树脂对葛根素的吸附与解吸性能。通过单因素试验、正交试验与验证实验,优化树脂分离纯化葛根素的工艺技术参数。结果表明:NKA-2树脂吸附效果最好,静态饱和吸附量可达184.9mg/g,在乙醇体积分数50%时的解吸率达90.86%;采用NKA-2树脂分离纯化葛根素的的最优参数为:上样液质量浓度18mg/mL,乙醇体积分数60%,洗脱速率1BV/h,此时葛根素的得率可达90.06%,纯度可达82.18%。该工艺具有较好的实用性与参考价值。
Macrosporous resin types D101,X-5,HD-101,D14,S-8,HC-D301,HD-8,D141,HPD100,HPD500,AB-8 and NKA-2 were compared for their adsorption and desorption capacities for the purification of puerarin from a crude extract of RadixPuerariae.NKA-2 was found to have the strongest ability to adsorb puerarin with a static saturated adsorption quantity of 184.9 mg/g and a desorption percentage of 90.86% by 50% ethanol.The optimal conditions for purifying puerarin with NKA-2,as determined by orthogonal array design,were 18 mg/mL,60% ethanol and 1 BV/h for sample concentration,desorbent and desorption flow rate,respectively.Under the optimized conditions,the recovery and purify of puerarin were 90.06% and 82.18%.The optimized process has potential practical applications for the purification of puerarin.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第16期89-92,共4页
Food Science
基金
湖南省教育厅科研项目(11C0668)
"十二五"国家科技支撑计划项目(2011BAD10B00)
关键词
大孔吸附树脂
葛根素
纯化
工艺参数
macroporous resin
puerarin
purification
process parameters
作者简介
李适（1981-），女，讲师，硕士，研究方向为植物功能成分化学。E—mail：lishidodo@163．com
通信作者：傅冬和（1967-），女，教授，博士，研究方向为植物功能成分化学。E-mail：fu7879@yahoo．com．cn
