摘要
建立了采用配位色谱柱从葛根素浸膏中分离纯化葛根素的方法。以铜离子为中心离子,制备了中心离子含量为7%的配位色谱柱。样品上样于配位色谱柱后,以氯仿-甲醇(体积比为10∶1)混合溶剂洗脱,得到了较纯的葛根素,较之用传统的硅胶色谱柱纯化,纯度提高了11%,回收率提高了12%,且柱容量提高了两倍。配位色谱改变了葛根素在传统硅胶柱上的洗脱顺序,对目标物质的分辨率比传统硅胶色谱柱高。
Puerarin, an isoflavone compound, is an important ingredient in traditional Chinese medicine, which has the good medical effects on hypertension and angina. A new type of separation and purification method for isolating puerarin was developed using chelate complex chromatography. The stationary phase was prepared with silica gel containing 7% of Cu（OAc） 2 in the chloroform. After the chloroform was evaporated, it was used as chelate complex chromatographic stationary phase to purify puerarin extractive. Pure puerarin was obtained when a mixture of chloroform and methanol （ 10： 1, v/v） was used as eluent. In comparison with a common silica gel column, the chelate complex chromatographic column was more convenient and efficient. The purity and recovery of puerarin, and column capacity were improved 1196, 12% and 200%, respectively. Under the optimized experimental conditions, the purity of pueratin could be as high as 93% with a recovery of 92%.
出处
《色谱》
CAS
CSCD
北大核心
2006年第5期482-485,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目(No.20136020)
关键词
配位色谱
铜离子
分离纯化
葛根素
葛根素浸膏
chelate complex chromatography
copper ion
isolation and purification
puerarin
puerarin extractive
作者简介
潘见，男，博士，教授．
通讯联系人：袁传勋，男，博士，研究员，在安徽省文物考古研究所工作，Tel：（0551）2901821—8205，E-mail：ycx608@sohu．com．
