摘要
本研究旨在探讨犀角地黄汤对脂多糖引起大鼠发热的退热效果及其机制。120只SD大鼠,随机分为4组,对照组、脂多糖组、阿司匹林组和犀角地黄汤组,每组30只。脂多糖组、阿司匹林组和犀角地黄汤组腹腔注射100μg·kg-1脂多糖复制大鼠发热模型,2h后,阿司匹林组和犀角地黄汤组分别灌服阿司匹林(0.04g·mL-1,按体重以10mL·kg-1给药)和犀角地黄汤(1.6g·mL-1,按体重以10μg·kg-1给药);对照组和脂多糖组灌服同体积的生理盐水。检测各组动物基础体温和攻毒给药后每小时肛温,并在2、3、5、8h分别从每组中随机选取6只大鼠断头处死,采取下丘脑组织,检测其IL-1β和PGE2的变化。结果表明,与脂多糖组比较,犀角地黄汤能显著缓解脂多糖引起的大鼠体温升高,且在用药后2h显著降低脂多糖引起的IL-1β(P<0.05)和PGE2(P<0.05)的升高,用药后3h显著降低IL-1β的升高(P<0.05)。说明犀角地黄汤能明显抑制脂多糖引起的大鼠发热,其初期的退热作用机制可能与抑制下丘脑IL-1β的生成,进而抑制发热介质PGE2释放有关。
The aim of this study was to investigate the effect and mechanisms of Xijiao Dihuang Decotion（XJDH）on Lipopolysaccharide （LPS） -induced fever in rats. One hundred and twenty rats were randomly allotted into four groups： Control group, LPS group, Aspirin group and XJDH group. There were 30 rats in each group. LPS group, Aspirin group and XJDH group were reproduced by intraperitoneal injection of LPS 100 μg · kg 1 , then Aspirin group and XJDH group were drenched with Aspirin （0.04 g · mL-1 , 10 mL ·kg 1 in drug delivery according to body weight） and XJDH （1.6 g ·mL -1,10 μg · kg-1 in drug delivery according to body weight） respectively;Control group and LPS group were drenched with saline. The basal body temperature was measured before LPS was injected and then 8 h later, 2 h after administration, 6 Hypo- thalamus samples were collected in each group at the point of 2, 3, 5 and 8 h. IL-1β and PGE2were measured. The results showed that, compared with LPS group, the temperature in XJDH group were significantly improved; Compared with the control group, IL -1β of LPS group was significantly increased （P〈0.05） at 2 h and 3 h, PGE2of LPS group was significantly increased （P〈0.05） at 2 h; Compared with LPS group, XJDH significantly lowered the levels of IL-1β and PGE2 （P〈0.05）. The above results suggest that XJDH significantly inhibited LPS-induced fever in Rats. The mechanism of initial antipyretic may be related to the inhibition of IL-1β in Hypo- thalamus, and reduce the release of PGE2
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2013年第7期1155-1159,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
北京市教委科技面上项目(KM201210020008)
国家自然科学基金(31101850)
北京市自然科学基金(6132007)
科研基地-科技创新平台(PXM2012_014207_000013)
北京农学院2012年度科研质量提高经费资助(PXM2012_014207_000010)
北京市教委人才强教深化计划-学术创新团队(PHR201007142)
北京市属高等学校人才强教计划资助项目(PHR201107134)
作者简介
董虹（1978），女，汉族，四川绵竹人，副教授，博士，主要从事中兽药研发及分子机理研究，E—mail：donghong523@163．com，Tel：010—80799480
通信作者：穆祥，教授，E—mail：muxiang1109@sina．com
