摘要
目的 探讨GATA-3表达在支气管哮喘(哮喘)小鼠发病机制中的作用。方法 12只C57BL/6小鼠随机均分为2组,对照组(A组)、哮喘组(B组)。B组建立哮喘模型,各组小鼠HE染色切片观察小鼠气管、肺组织病理变化,免疫组化测定肺组织中GATA-3表达及含量。结果 在同一视野对照组支气管黏膜下未见炎性细胞,哮喘组可见大量炎性细胞浸润。 免疫组化染色A组GATA-3表达(6±0.5),B组GATA-3表达(64±0.2)。结论 哮喘小鼠肺部存在GATA-3高表达,支气管 哮喘小鼠肺组织存在多种炎性细胞浸润,其机制之一与支气管小鼠肺组织中GATA-3高表达有关。
Objective To investigate the expressions of GATA-3 in asthmatic mice and its significance.Methods C57BL/6 mice were randomly divided into two groups:control group ( n = 6,group A) ,and asthmatic model group( n = 6,group B) .The asthmatic model was developed in group B by ovalbumin(OVA) absorbed in to aluminum hydroxide. The pathological changes in trachea and lung tissue were examined. The expression of GATA-3 in the lung was analyzed by immohistochemistry. Results Microscopically, inflammatory cells were found in group A,but a great deal of inflammatory cell infiltration was visualied in gruup B. Immumohistochemistry showed that the expression of GATA-3 in group B(64±0.2) was stronger than that in group A(6±0.5) .Conclusion Asthmatic mice exhibit high expression of GATA-3, and copious inflammtory cell infiltration in lung tissue, which is related to the high expression of GATA-3 in the murine asthmatic model.
出处
《武警医学》
CAS
2004年第4期258-261,共4页
Medical Journal of the Chinese People's Armed Police Force
基金
国家自然科学基金项目(301704116)
