摘要
分别在水溶液中和 Ｗ ／Ｏ型 ＣＴＡＢ／正戊醇／异辛烷 ／水微乳液体系中研究了测定ＳＯＤ酶活性的邻苯三酚法，发现在反向微乳液中，“小水池”的低介电常数和ｐＨ抑制了底物超氧化物阴离子Ｏ２－·的产生．只有提高外加缓冲液的ｐＨ或“小水池”的水化度，才能得到所需浓度的Ｏ２－·，从而初步解决了在微乳液中ＳＯＤ作用废物的产生问题，为在微乳液中准确测定ＳＯＤ酶活性创造了条件．
The pyrogallic method for determining the activity of SOD is studied in the aqueous solution and the CTAB/n -pentanol/n -octane/water microemulsion, respectively. It is found that the low pH and the dielectric constant inhibit the generation of the substrate O2-· in the reverse microemulsion which is later improved by increasing the above two factors. It solves the problem of the generation of O2- which create a new way for the determination of the activity of SOD in the microenvironment.
出处
《分子科学学报》
CAS
CSCD
2000年第2期65-69,共5页
Journal of Molecular Science
基金
国家自然科学基金!29903006
