摘要
目的:考察西藏昌都、那曲当归根与道地药材岷县当归根挥发油、总黄酮含量和体外抗氧化活性,为当归资源的开发利用提供药源和理论依据。方法:以《中华人民共和国药典》2015版中挥发油测定法通则2204乙法为依据,提取各地产当归根中的挥发油并进行含量对比;采用乙醇浸提,结合加热回流提取各地当归根中的总黄酮;采用NaNO_(2)-AlCl_(3)比色法,以芦丁为对照品,对当归中总黄酮进行含量测定。采取浸泡、超声、过滤、烘干、溶解、浓缩得乙醇提取物,以总抗氧化能力及对DPPH自由基、超氧阴离子和羟基自由基的清除能力综合评价各地产当归的体外抗氧化活性。结果:与道地药材岷县当归根比较,西藏昌都、那曲当归根挥发油含量均较低(***P<0.001),且未达到“药典”标准要求;然而,西藏昌都、那曲当归根总黄酮含量分别为3.076 mg·g^(-1)、2.681 mg·g^(-1),与岷县当归根中总黄酮含量1.715 mg·g^(-1)比较,含量显著高且差异具有统计学意义(***P<0.001)。与岷县当归根比,西藏昌都、那曲当归根的总抗氧化能力及对DPPH自由基、超氧阴离子和羟基自由基的清除能力均较强,但差异不具有统计学意义。结论:西藏昌都、那曲当归根挥发油含量低而总黄酮含量高,均表现出显著的体外抗氧化活性,表明西藏当归作为药用资源可进一步开发利用。
Objective:To investigate the volatile oil,total flavonoids content and in vitro antioxidant activity of angelica root in Changdu,Naqu in Tibet and the authentic medicinal material Minxian inland,in order to provide medicinal sources and theoretical basis for the development and utilization of angelica resources.Methods:Based on the 2204 B method of the General Principles of Determination of Volatile Oils in Chinese Pharmacopoeia 2015 Edition,the volatile oils in the roots of Angelica sinensis were extracted and compa red.The total flavonoids in the roots of Angelica sinensis were extracted by ethanol extraction,combined with heating and refluxing;Using NaNO_(2)-AlCl_(3)colorimetric method,with rutin as the reference substance,the content of total flavonoids in Angelica sinensis was determined.The ethanol extract was obtained by soaking,ultrasonication,filtration,drying,dissolving and concentration,and the in vitro antioxidant activity of Angelica sinensis was comprehensively evaluated for total antioxidant capacity,DPPH free radical,superoxide anion and hydroxyl radical scavenging ability.Results:Compared with the native medicinal materials of Minxian angelica root in the mainland,the volatile oil content of Tibetan Qamdo and Naqu Angelica roots were lower(***P about 0.001),and did not meet the requirements of the pharmacopoeia;however,the total flavonoids of Tibetan Qamdo and Naqu Angelica roots The contents were 3.076 and 2.681 mg·g^(-1),respectively.Compared with the total flavonoid content of 1.715 mg·g^(-1)in Angelica root of Minxian County,the content was significantly higher and the difference was statistically significant(***P about 0.001).Compared with Minxian angelica roots,the total antioxidant capacity,the scavenging ability of DPPH free radicals,s uperoxide anions and hydroxyl free radicals of Tibetan Qamdo and Naqu angelica roots were stronger,but the differences were not statistically significant.Conclusion:Tibetan Qamdo and Naqu Angelica roots have low volatile oil content and high total flavonoid content,and both show significant in vitro antioxidant activity,indicating that Tibetan Angelica root can be further developed and utilized as a medicinal resource.
作者
宋丹
陈依春
姜钊
江晨雨
李沐蓉
王玉蓉
赵杰志
色里玛
李捷
SONG Dan;CHEN Yi-chun;JIANG Zhao;JIANG Chen-yu;LI Mu-rong;WANG Yu-rong;ZHAO Jie-zhi;SE Li-ma;LI Jie(Tibet Nationalities University School of Medicine,Xianyang 712082,China;Engineering Research Center for Tibetan Medicine Testing Technology Ministry of Education,Xianyang 712082,China;Tibet Ganlu Tibetan Medicine limited company,Lhasa 850000,China)
出处
《中国民族医药杂志》
2022年第6期37-41,共5页
Journal of Medicine and Pharmacy of Chinese Minorities
基金
国家自然科学基金项目(编号32160165)
西藏自治区自然科学基金项目(编号XZ 2018 ZRG-86(Z))
关键词
西藏昌都当归
西藏那曲当归
挥发油
总黄酮
体外抗氧化活性
Tibet Changdu Angelica
Tibet Naqu Angelica
Volatile oil
Total flavonoids
Antioxidant activity in vitro
作者简介
宋丹(1990-),女。研究方向:藏药材在高原病中的干预机制研究。E-mail:dsong@xzmu.edu.cn;通讯作者:李捷(1985-),女,硕士,实验师。研究方向:藏药材开发利用及抗高原病机制研究。E-mail:lijienba@163.com。
