摘要
类胡萝卜素对蛹虫草子实体形成有很大影响。为了探讨类胡萝卜素与蛹虫草子实体形成的关系,选取三种典型菌落形态的蛹虫草菌株,研究其在光照黑暗交替条件下不同菌落形态和类胡萝卜素含量对子实体形成的影响。采用酸热法和吸收光谱法提取测定类胡萝卜素含量。结果表明三类蛹虫草在沙氏平板上均有可形成子实体的菌株,说明形态与子实体形成无直接关系。类胡萝卜素含量在3000μg/g以下的菌株在沙氏培养基上无子实体形成,3000~3500μg/g有子实体形成平均为2个/cm2,3500~6500μg/g菌株子实体形成能力较强平均为7个/cm2,含量在6500μg/g以上菌株形成子实体平均在5个/cm2,全黑暗培养菌株未检测到类胡萝卜素,也无子实体形成。
Carotenoids are the key factors affecting the formation of fruit bodies of Cordyceps militaris. Three type strains Cordyceps militaris which had typical colony morphology were selected. The influence of conditions such as light and dark cycle,different colony morphology on the formation of the fruit body and contents of carotenoids was investigated. Carotenoids were extracted by hydrochloric acid (HCI) with heating (by boiling water) and detected by absorption spectroscopy. The result was that the strains had ability to product fruit bodies in three morphology differently, the formation of fruit body and colony morphology were not related. The fruit body could not be formed as the carotenoid content of less than 3000μg/g. However, the rate of formation of fruit body was 2,7,5 each square centimetre,as the carotenoids contents of 3000-3500μg/g, 3500-6500μg/g, more than 6500μg/g, respectively. On the contrary, neither the fruit body and the carotenoids could be detected under the dark condition.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第6期194-196,201,共4页
Science and Technology of Food Industry
关键词
蛹虫草
类胡萝卜素
子实体
Cordyceps militaris(L.) Link
carotenoids, fruit body
作者简介
胡久平(1986一),男,在读硕士,研究方向:微生物活性。