摘要
中药材中活性成分的含量是中药品质优劣的关键指标。本文建立了香薷中总黄酮和3种主要黄酮的分析方法,探索不同部位黄酮含量的差异,为香薷黄酮类成分的开发利用奠定基础。结果表明优化后的分光光度法对香薷总黄酮测定具有专一性、稳定性。高效液相色谱测定木犀草素、芹菜素和黄芩素-7-甲醚的方法在测定范围内表现出良好的线性关系(r>0.999);方法的回收率在98.77%～102.13%之间,RSD均小于5%;不同部位总黄酮的含量由高到低依次为:苞>尖>茎>根,且江西产香薷总黄酮的含量高于浙江产的香薷。这提示在工业生产上提取香薷黄酮类物质时尽量选择道地药材,同时可考虑弃去根部及茎的下端,以便提高浸提效率,增加经济效益。本试验建立的香薷中总黄酮和游离黄酮的测定方法准确、稳定,明确了香薷中总黄酮主要富集在花苞及附近,为进一步提高香薷资源的开发利用提供了理论依据和科学指导。
The content of active constituents in Chinese herbal medicine is a key indicator for judging their qualities. In this study, the methods were established for analyzing total and three major flavonoids in Mosla chinensis, respectively, and the differences of the flavonoid contents in different tissues were also compared, which will favour the exploitation of the flavonoids in M. chinensis. The results showed that the optimized spectrophotometry for the determination of total flavonoid content was specific and stable, and the HPLC method for the analysis of luteolin, apigenin and negletein presented a good linear relationship （ r 〉 0. 999） in the range of the determination and the recovery ranges were 98.77% - 102.13% with less than 5% RSDs. The study also found that the content of total flavonoids in different parts of M. chinensis from high to low was bud 〉 tip 〉 stem 〉 root, and the total flavonoid content in M. chinensis from Jiangxi was higher than that from Zhejiang. These prompt that genunie medicinal materials should be selected for the industrial production of the flavonoids from M. chinensis as more as possible, and the root and the lower end of stem may be considered to discard, so as to improve the extraction efficiency and increase economic benefits. In brief, the developed methods are accurate and steady for detecting total and three major flavonoids in M. chinensis, and we also confirmed total flavonoids were mainly enriched in and near the bud of M. chinensis, which would provide a theoretical basis and scientific guidance for further exploiting the resources in M. chinensis.
出处
《植物研究》
CAS
CSCD
北大核心
2013年第2期252-256,共5页
Bulletin of Botanical Research
基金
国家自然科学基金(31100502)
教育部高校博士点专项基金(20114320120004)
作者简介
蒋华军（1985-），男，硕士研究生，主要从事功能产品开发与评价。
通信作者：E-mail：larkin-liu@163．com.
