摘要
目的采用顶空气相色谱法测定人参茎叶皂苷中大孔树脂残留物的质量浓度。方法色谱柱初始温度60℃,保持22min,以50℃/min升至200℃,保持6min,氢火焰离子检测器温度为260℃,进样口温度220℃;用质量分数为40%的N,N-二甲基甲酰胺作溶剂,载气为氮气。结果正己烷、苯、甲苯、对二甲苯、苯乙烯、对二乙基苯6种大孔树脂残留物在各自的质量浓度范围内呈良好的线性关系,且回收率符合要求。结论该方法操作简便快速,灵敏度高,准确度好,可作为人参茎叶总皂苷中大孔树脂有机溶剂残留量的测定方法。
Objective To establish a headspace gas chromatography method for the determination of macroporous resin residues in the saponins extracted from the leaves of Panax ginseng.Methods The initial column temperature was 60 ℃（for 22 min）,and increased to 200 ℃ at a rate of 50 ℃/min（for 6 min）;The injector temperature was set at 220 ℃ and the temperature of FID detector was set at 260 ℃;The solvent was 40% N,N-dimethyl formamide（DMF）;The carrier gas was nitrogen.Results All the residues including n-hexane,benzene,toluene,1,4-xylene,styrene,and 1,4-diethyl-benzene had good linearities and the recoveries in their concentration ranges,respectively.Conclusion The method is simple,quick,accurate,and sensitive.It could be used for the determination of macroporous resin residues in the saponins from the leaves of P.ginseng.
出处
《药物评价研究》
CAS
2013年第1期48-50,共3页
Drug Evaluation Research
关键词
人参茎叶皂苷
大孔吸附树脂
残留物
顶空气相色谱法
saponins in leaves of Panax ginseng
macroporous resin
residues
headspace gas chromatography
作者简介
张蓓，女，副主任药师。Tel：（0431）85101325E-mail：gretazpll@163．com
