摘要
目的：测定荞麦不同栽培种、植株不同部位以及不同商品苦荞茶中的总黄酮含量。方法：采用UV法测定总黄酮含量;通过回流法提取,NaNO3-Al（NO3）3法显色测定样品吸光度,以芦丁为标准溶液计算求得总黄酮含量。结果：芦丁在0.003006~0.1503mg/mL浓度范围内线性关系良好,R2=0.9999;平均回收率为97.9%,RSD为1.95%。结论：不同栽培种荞麦总黄酮含量测定中,苦荞的总黄酮含量在13.67~21.56mg/g之间,甜荞总黄酮含量在5.79~7.23mg/g之间,苦荞总黄酮含量总体高于甜荞;荞麦植株不同部位中叶的总黄酮含量最高,最高达到68.30mg/g;商品苦荞茶中,所测商品总黄酮含量在6.96~16.78mg/g之间,较苦荞种子中总黄酮含量有一定程度减少。该方法简便快捷,结果准确,重现性好,适用于荞麦和商品苦荞茶中总黄酮含量的测定。
Objective：To determine the content of total flavones in the different kinds of buckwheat,different parts of buckwheat and buckwheat goods. Method：UV spectrophotometry for determination of total flavonoids was used. Using Na （NO 3 ） -Al （NO 3 ） 3 to detect the absorbance of samples and then count the total flavonoids. Results：The linear range of rutin was 0.003006~0.1503mg/mL （R 2 =0.9999） with average recovery of 97.9% （RSD 1.95% ）. Conclusion：In different cultivated species of buckwheat,the concentration of total flavonoids of Fagopyrum tataricum（L.） Gaerth was 13.67~21.56mg/g,and that of F. esculentum Moench was 5.79~7.23mg/g. In different parts of buckwheat,the concentration of total flavonoids of leaves was the highest,up to 68.30mg/g. For buckwheat tea,the concentration of total flavonoids was 6.96~16.78mg/g,but they were lower than that of buckwheat seeds. This method was simple,accurate and reproducible for determination of total flavonoids of buckwheat and buckwheat goods.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第2期58-60,共3页
Science and Technology of Food Industry
基金
西南民族大学研究生创新科研项目(CX2013SZ21)
国家科技支撑计划项目(2012BAI27B07)
四川省科技支撑计划(2011SZ0233)
现代农业产业技术体系建设专项资金资助(CARS-08-D-3)
四川中医药管理局课题(2010-78)
关键词
荞麦
苦荞茶
总黄酮
含量测定
buckwheat
buckwheat tea
total flavonoids
content determination
作者简介
王静霞（1988-），女，硕士研究生，研究方向：民族药物；
黄艳菲（1986-），女，硕士研究生，研究方向：民族药物。
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