摘要
本试验选取了miR-1、miR-133、miR-24、miR-122、miR-103、miR-143、let7 7个miRNAs,旨在对其表达量及靶基因表达谱进行分析。选用4只绒山羊的背部和后腿肌肉提取RNA,反转录后,利用SYBR荧光定量PCR来检测各miRNAs的表达量,并采用靶基因指纹图谱(MTFP)技术获得各miRNAs的靶基因表达谱。结果表明,miR-NA表达量依次为:miR-1>miR-133>miR-24>let7>miR-143>miR-103>miR-122,其中miR-1和miR-133表达规律相似,且这7个miRNAs在成羊中的表达量均高于羔羊;此外,miRNAs表达量的差异还受性别影响,且miR-NA表达量与靶基因个数及其表达量之间具有相关性。对5个miRNAs的10个靶基因测序并比对,表明靶基因分别编码与信号转导、细胞周期等相关的蛋白。通过这7个miRNAs与其靶基因表达的分析,揭示了肌肉发育相关miRNAs及脂肪发育相关miRNAs在绒山羊骨骼肌中的异同,有利于绒山羊肉质基因的研究。
Seven muscle and adipose related miRNAs, including miR-1, miR-133, miR-24, miR- 122, miR-103, miR-143 and let7 were selected to analyze their expression and target expression profiles. Muscles RNA from back and hind leg muscle of 4 cashmere goats were extracted, after reverse-transcription, SYBR TaqMan PCR was used to detect gene expression, and miRNA tar- get expression profiles were got by PAGE electrophoresis and MiRNA Targets Figure Print （MTFP） technique. The result showed that the expression of miRNA were as follows：miR-l〉 miR-133〉miR-24〉let7〉miR-143〉miR-103〉miR-122, the expression patterns of miR-1 and miR-133 were similar,and the expression levels of all miRNAs in adult goat were higher than that in lamb; moreover, the difference of miRNAs expression levels were also affected by gender, and they had relationship with number and expression levels of target genes. 10 target genes in 5 miRNAs were sequenced and Blast annotation, which coded proteins related to signal transduc- tion, ceil cycle, and so on. The expression analysis of 7 miRNAs and their target genes showed differences and similarities between muscle development related miRNAs and adipose develop- ment related miRNAs in cashmere goat skeletal muscle, which were favorahle to the research for meat quality related genes in cashmere goat.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第10期1539-1546,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科技支撑项目(2011BAD28B05)
国家自然科学基金(30960246)
现代农业产业技术体系建设专项资金(CARS-40-05)
公益性行业(农业)科研项目(201003019)
关键词
MIRNA
靶基因
表达
绒山羊
骨骼肌
miRNA
target genel expression
cashmere goat
skeletal muscle
作者简介
韩志玲（1986-），女，山西朔州人，硕士生，主要从事动物遗传育种与繁殖研究，E-mail：hanzhilingl986@163．com
赵德超（1987-），男，河北沧县人，硕士生，主要从事动物遗传育种与繁殖研究，E-mail：zhaodecha019870324@163．com。
通讯作者：张文广，E—mail：atcgnmbi@yahoo．com．cn
李金泉，E-mail：lijinquan-nd@126．com
